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Postnatal developmental changes in Ca2+ homeostasis in supraoptic magnocellular neurons
Cited 14 time in
Web of Science
Cited 16 time in Scopus
- Authors
- Issue Date
- 2006-10-03
- Publisher
- Elsevier
- Citation
- Cell Calcium. 2007 May;41(5):441-50. Epub 2006 Sep 27.
- Keywords
- Animals ; Animals, Newborn ; Caffeine/pharmacology ; Calcium/*metabolism ; Calcium Signaling/drug effects ; Male ; Neurons/cytology/drug effects/enzymology/*metabolism ; Rats ; Rats, Sprague-Dawley ; Sarcoplasmic Reticulum Calcium-Transporting ATPases/metabolism ; Sodium/pharmacology ; Sodium-Calcium Exchanger/metabolism ; Supraoptic Nucleus/*cytology/*growth & development ; Thapsigargin/pharmacology ; Homeostasis/drug effects
- Abstract
- Supraoptic magnocellular neurons (SMNs) undergo dramatic changes in morphological and electrical properties during postnatal development. We investigated the developmental change in Ca2+ homeostasis in SMNs. The decay rate of Ca2+ transients markedly increased during the third postnatal week (PW3) to an adult level. This increase in the Ca2+ decay rate was paralleled by hypertrophy of the SMN somata. Activity of Na+/Ca2+ exchanger (Na/CaX) and sarcoendoplasmic reticulum Ca2+-ATPase (SERCA) was quantified as a decrement in the Ca2+ decay rate caused by extracellular [Na+] reduction and that by thapsigargin, respectively. SERCA activity was negligible during PW2, and markedly increased during PW3. SERCA activity and soma size remained stable thereafter. Na/CaX activity was a major Ca2+-clearance mechanism (CCM) during PW2, increased further during PW3, but was negligible in mature SMNs (PW10). In parallel with the decrease in Na/CaX activity, endogenous Ca2+ buffering capacity declined, resulting that the apparent Ca2+ decay rate remained relatively constant between PW4 and PW10. Replacement of intracellular K+ with Li+ had no effect on Na/CaX activity, suggesting that NCX rather than NCKX comprises Na/CaX. These findings indicate a developmental shift in the balance of CCMs from Ca2+ extrusion via NCX toward Ca2+ sequestration into endoplasmic reticulum via SERCA.
- ISSN
- 0143-4160 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17010427
https://hdl.handle.net/10371/29264
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