Publications
Detailed Information
Hypoxia inhibits the SDF-1-dependent migration of human leukemic cell line HL-60 via blocking of Akt activation
Cited 6 time in
Web of Science
Cited 6 time in Scopus
- Authors
- Issue Date
- 2007-10-24
- Publisher
- Elsevier
- Citation
- Biochem Biophys Res Commun. 2007 Dec 14;364(2):388-94. Epub 2007 Oct 15.
- Keywords
- Cell Hypoxia ; Chemokine CXCL12/*metabolism ; Chemotaxis ; Chromones/pharmacology ; Enzyme Inhibitors/pharmacology ; Flavonoids/pharmacology ; HL-60 Cells ; Humans ; Hypoxia-Inducible Factor 1, alpha Subunit/biosynthesis ; Morpholines/pharmacology ; Phosphorylation ; Proto-Oncogene Proteins c-akt/antagonists & inhibitors/*metabolism ; Receptors, CXCR4/biosynthesis
- Abstract
- Hypoxia is known to regulate the expression of genes involved in the migration of various cell types. Although many studies have shown that hypoxia increases cell migration, it still remains unclear whether hypoxia could modulate the stromal cell derived factor-1 (SDF-1)-dependent migration of leukemic cell. Herein, we demonstrated that the SDF-1-dependent migration of HL-60, was reduced under hypoxia with no comparable decrease of CXC-type chemokine receptor CXCR4, a cognate receptor for SDF-1. Furthermore, we showed that migration toward SDF-1 was reduced by inactivation of either serine/threonine kinase Akt or extracellular signal regulated kinase Erk, which was confirmed by selective pathway inhibitor LY294002 and PD98059. In our results, phosphorylation of Erk was increased under hypoxia, but phosphorylation of Akt was attenuated on the contrary. These results led us to conclusion that hypoxia could inhibit the SDF-1-dependent migration of HL-60 via blocking of Akt activation.
- ISSN
- 1090-2104 (Electronic)
- Language
- English
- URI
- http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-4PX127K-9&_user=10&_rdoc=1&_fmt=&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=21b0704be66470e71da7f0936b25e444
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17950696
https://hdl.handle.net/10371/29397
- Files in This Item:
- There are no files associated with this item.
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.