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Anti-inflammatory effects of 8-hydroxy-2'-deoxyguanosine on lipopolysaccharide-induced inflammation via Rac suppression in Balb/c mice
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Seongwon | - |
dc.contributor.author | Choi, Hyun-Ho | - |
dc.contributor.author | Lee, Sun-Hye | - |
dc.contributor.author | Ko, Seong-Hee | - |
dc.contributor.author | You, Ho-Jin | - |
dc.contributor.author | Ye, Sang-Kyu | - |
dc.contributor.author | Chung, Myung-Hee | - |
dc.date.accessioned | 2010-01-11T07:42:17Z | - |
dc.date.available | 2010-01-11T07:42:17Z | - |
dc.date.issued | 2007-11-27 | - |
dc.identifier.citation | Free Radic Biol Med. 2007 Dec 15;43(12):1594-603. Epub 2007 Sep 6. | en |
dc.identifier.issn | 0891-5849 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18037125 | - |
dc.identifier.uri | https://hdl.handle.net/10371/29411 | - |
dc.description.abstract | Recently, we observed that 8-hydroxyguanosine triphosphate and 8-hydroxy-2'-deoxyguanosine (oh(8)dG) inactivate Rac and consequently down-regulate the Rac-linked NADPH oxidase, iNOS, and Cox2. Based on these observations, we tested whether oh(8)dG has anti-inflammatory activity in vivo in lipopolysaccharide (LPS)-treated mice. LPS (1 mg/kg, ip)-treated mice exhibit marked inflammatory responses, including increases in proinflammatory cytokines (TNF-alpha, IL-6, IL-18, and IL-12p70) in serum and infiltration of neutrophils, increased translocation of NF-kappaB p50 from the cytosol to the nucleus, and phosphorylation of c-Jun in lung tissues. Mice were pretreated with oh(8)dG (up to 60 mg/kg, ip) 4 h before LPS injection, and this pretreatment dose-dependently inhibited the inflammatory responses; the inhibitions observed with 60 mg/kg oh(8)dG were statistically significant. At the same time, oh(8)dG pretreatment inactivated Rac in lung tissues. Oh(8)dG pretreatment (50 mg/kg, ip) also significantly protected against LPS-induced septic death. Furthermore, oh(8)dG was more effective than acetyl salicylic acid in inhibiting these inflammatory responses. 8-Hydroxyguanosine also had some effect but was much weaker than oh(8)dG. The effects of normal nucleosides (dG, G, and A) were negligible or not significant. These results support an anti-inflammatory activity for oh(8)dG, which could be ascribed to its Rac-inactivating action. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Animals | en |
dc.subject | Anti-Inflammatory Agents, Non-Steroidal/pharmacology | en |
dc.subject | Cytokines/blood | en |
dc.subject | Deoxyguanosine/*analogs & derivatives/pharmacology | en |
dc.subject | Free Radicals/metabolism | en |
dc.subject | Inflammation/chemically induced/metabolism/*prevention & control | en |
dc.subject | JNK Mitogen-Activated Protein Kinases/metabolism | en |
dc.subject | Lipopolysaccharides/toxicity | en |
dc.subject | Lung/drug effects/metabolism/pathology | en |
dc.subject | Male | en |
dc.subject | Mice | en |
dc.subject | Mice, Inbred BALB C | en |
dc.subject | NF-kappa B p50 Subunit/metabolism | en |
dc.subject | Neutrophils/drug effects/pathology | en |
dc.subject | Peroxidase/metabolism | en |
dc.subject | rac GTP-Binding Proteins/*antagonists & inhibitors | en |
dc.title | Anti-inflammatory effects of 8-hydroxy-2'-deoxyguanosine on lipopolysaccharide-induced inflammation via Rac suppression in Balb/c mice | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 최성원 | - |
dc.contributor.AlternativeAuthor | 최현호 | - |
dc.contributor.AlternativeAuthor | 이선혜 | - |
dc.contributor.AlternativeAuthor | 고성희 | - |
dc.contributor.AlternativeAuthor | 유호진 | - |
dc.contributor.AlternativeAuthor | 예성규 | - |
dc.contributor.AlternativeAuthor | 정명희 | - |
dc.identifier.doi | 10.1016/j.freeradbiomed.2007.08.022 | - |
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