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Nuclear factor-kappaB activation correlates with better prognosis and Akt activation in human gastric cancer

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dc.contributor.authorLee, Byung lan-
dc.contributor.authorLee, Hye Seung-
dc.contributor.authorJung, Jieun-
dc.contributor.authorCho, Sung Jin-
dc.contributor.authorChung, Hee-Yong-
dc.contributor.authorKim, Woo Ho-
dc.contributor.authorJin, Young-Woo-
dc.contributor.authorKim, Chong Soon-
dc.contributor.authorNam, Seon Young-
dc.date.accessioned2010-01-12T01:46:51Z-
dc.date.available2010-01-12T01:46:51Z-
dc.date.issued2005-04-09-
dc.identifier.citationClin Cancer Res. 2005 Apr 1;11(7):2518-25.en
dc.identifier.issn1078-0432 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15814628-
dc.identifier.urihttp://hdl.handle.net/10371/29520-
dc.description.abstractPURPOSE: Because the biological significance of constitutive nuclear factor-kappaB (NF-kappaB) activation in human gastric cancer is unclear, we undertook this study to clarify the regulatory mechanism of NF-kappaB activation and its clinical significance. EXPERIMENTAL DESIGN: Immunohistochemistry for NF-kappaB/RelA was done on 290 human gastric carcinoma specimens placed on tissue array slides. The correlations between NF-kappaB activation and clinicopathologic features, prognosis, Akt activation, tumor suppressor gene expression, or Bcl-2 expression were analyzed. We also did luciferase reporter assay, Western blot analysis, and reverse transcription-PCR using the SNU-216 human gastric cancer cell line transduced with retroviral vectors containing constitutively active Akt or the NF-kappaB repressor mutant of IkappaBalpha. RESULTS: Nuclear expression of RelA was found in 18% of the gastric carcinomas and was higher in early-stage pathologic tumor-node-metastasis (P = 0.019). A negative correlation was observed between NF-kappaB activation and lymphatic invasion (P = 0.034) and a positive correlation between NF-kappaB activation and overall survival rate of gastric cancer patients (P = 0.0228). In addition, NF-kappaB activation was positively correlated with pAkt (P = 0.047), p16 (P = 0.004), adenomatous polyposis coli (P < 0.001), Smad4 (P = 0.002), and kangai 1 (P < 0.001) expression. An in vitro study showed that NF-kappaB activity in gastric cancer cells is controlled by and controls Akt. CONCLUSIONS: NF-kappaB activation was frequently observed in early-stage gastric carcinoma and was significantly correlated with better prognosis and Akt activation. These findings suggest that NF-kappaB activation is a valuable prognostic variable in gastric carcinoma.en
dc.language.isoenen
dc.publisherAmerican Association for Cancer Researchen
dc.subjectAdolescenten
dc.subjectAdulten
dc.subjectAgeden
dc.subjectAged, 80 and overen
dc.subjectAndrostadienes/pharmacologyen
dc.subjectBlotting, Westernen
dc.subjectCell Line, Tumoren
dc.subjectChilden
dc.subjectChild, Preschoolen
dc.subjectChromones/pharmacologyen
dc.subjectEnzyme Activationen
dc.subjectFemaleen
dc.subjectGene Expression Regulation, Neoplastic/drug effectsen
dc.subjectGenetic Vectors/geneticsen
dc.subjectHumansen
dc.subjectImmunohistochemistryen
dc.subjectInfanten
dc.subjectInfant, Newbornen
dc.subjectLuciferases/genetics/metabolismen
dc.subjectMaleen
dc.subjectMiddle Ageden
dc.subjectMorpholines/pharmacologyen
dc.subjectNF-kappa B/analysis/genetics/*metabolismen
dc.subjectNeoplasm Stagingen
dc.subjectPrognosisen
dc.subjectProtein-Serine-Threonine Kinases/genetics/*metabolismen
dc.subjectProto-Oncogene Proteins/genetics/*metabolismen
dc.subjectProto-Oncogene Proteins c-akten
dc.subjectProto-Oncogene Proteins c-bcl-2/analysisen
dc.subjectRNA, Messenger/genetics/metabolismen
dc.subjectRecombinant Fusion Proteins/genetics/metabolismen
dc.subjectRetroviridae/geneticsen
dc.subjectReverse Transcriptase Polymerase Chain Reactionen
dc.subjectStomach Neoplasms/genetics/metabolism/*pathologyen
dc.subjectSurvival Analysisen
dc.subjectTissue Array Analysisen
dc.subjectTranscription Factor RelAen
dc.subjectTransfectionen
dc.subjectTumor Suppressor Proteins/analysisen
dc.titleNuclear factor-kappaB activation correlates with better prognosis and Akt activation in human gastric canceren
dc.typeArticleen
dc.contributor.AlternativeAuthor이병란-
dc.contributor.AlternativeAuthor이혜승-
dc.contributor.AlternativeAuthor정지은-
dc.contributor.AlternativeAuthor조성진-
dc.contributor.AlternativeAuthor정희용-
dc.contributor.AlternativeAuthor김우호-
dc.contributor.AlternativeAuthor진영우-
dc.contributor.AlternativeAuthor김종순-
dc.contributor.AlternativeAuthor남선영-
dc.identifier.doi10.1158/1078-0432.CCR-04-1282-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Anatomy (해부학전공)Journal Papers (저널논문_해부학전공)
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