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Direct application of AvaII PCR restriction fragment length polymorphism analysis (AvaII PRA) targeting 644 bp heat shock protein 65 (hsp65) gene to sputum samples
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Mun, Ho-Suk | - |
dc.contributor.author | Kim, Hyun-Ju | - |
dc.contributor.author | Oh, Eun-Ju | - |
dc.contributor.author | Kim, Hong | - |
dc.contributor.author | Park, Young-Gil | - |
dc.contributor.author | Bai, Gil-Han | - |
dc.contributor.author | Do, Junghwan | - |
dc.contributor.author | Cha, Chang-Yong | - |
dc.contributor.author | Kook, Yoon-Hoh | - |
dc.contributor.author | Kim, Bum-Joon | - |
dc.date.accessioned | 2010-01-12T01:54:37Z | - |
dc.date.available | 2010-01-12T01:54:37Z | - |
dc.date.issued | 2007-01-24 | - |
dc.identifier.citation | Microbiol Immunol. 2007;51(1):105-10. | en |
dc.identifier.issn | 0385-5600 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17237605 | - |
dc.identifier.uri | https://hdl.handle.net/10371/29539 | - |
dc.description.abstract | To evaluate the usefulness of the AvaII PRA method targeting 644-bp hsp65 gene for the direct detection of pathogenic mycobacteria from clinical specimens, we applied this method to 40 sputum samples and compared the results to those obtained by IS 6110 PCR. Although this method showed a sensitivity slightly lower than IS 6110 PCR (97.5% vs. 100%), it detected infections of M. avium complex (MAC) in two patients, which was not possible by IS 6110 PCR. We conclude that AvaII PRA is a highly effective method for directly detecting pathogenic mycobacteria in primary clinical specimens. | en |
dc.language.iso | en | en |
dc.publisher | Wiley-Blackwell | en |
dc.subject | Bacterial Proteins/*genetics | en |
dc.subject | Bacteriological Techniques | en |
dc.subject | Chaperonins/*genetics | en |
dc.subject | DNA, Bacterial/*genetics/metabolism | en |
dc.subject | Deoxyribonucleases, Type II Site-Specific/metabolism | en |
dc.subject | Humans | en |
dc.subject | Mycobacterium/genetics/*isolation & purification | en |
dc.subject | Polymerase Chain Reaction | en |
dc.subject | Sensitivity and Specificity | en |
dc.subject | Sputum/*microbiology | en |
dc.subject | DNA Fingerprinting | - |
dc.subject | Polymorphism, Restriction Fragment Length | - |
dc.title | Direct application of AvaII PCR restriction fragment length polymorphism analysis (AvaII PRA) targeting 644 bp heat shock protein 65 (hsp65) gene to sputum samples | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 문호숙 | - |
dc.contributor.AlternativeAuthor | 김현주 | - |
dc.contributor.AlternativeAuthor | 오은경 | - |
dc.contributor.AlternativeAuthor | 김홍 | - |
dc.contributor.AlternativeAuthor | 박영길 | - |
dc.contributor.AlternativeAuthor | 배길한 | - |
dc.contributor.AlternativeAuthor | 도중환 | - |
dc.contributor.AlternativeAuthor | 차창용 | - |
dc.contributor.AlternativeAuthor | 국윤호 | - |
dc.contributor.AlternativeAuthor | 김범준 | - |
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