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Detection and identification of Mycobacterium tuberculosis in joint biopsy specimens by rpoB PCR cloning and sequencing

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dc.contributor.authorYun, Yeo-Jun-
dc.contributor.authorLee, Keun-Hwa-
dc.contributor.authorHaihua, Lin-
dc.contributor.authorRyu, Yoon-Jong-
dc.contributor.authorKim, Bum-Joon-
dc.contributor.authorLee, Young-Ho-
dc.contributor.authorBaek, Goo-Hyun-
dc.contributor.authorKim, Hee-Joong-
dc.contributor.authorChung, Moon-Sang-
dc.contributor.authorLee, Myung-Chul-
dc.contributor.authorLee, Sang-Hoon-
dc.contributor.authorChoi, In-Ho-
dc.contributor.authorCho, Tae-Jun-
dc.contributor.authorChang, Bong-Soon-
dc.contributor.authorKook, Yoon-Hoh-
dc.date.accessioned2010-01-12T02:24:10Z-
dc.date.available2010-01-12T02:24:10Z-
dc.date.issued2005-01-07-
dc.identifier.citationJ Clin Microbiol. 2005 Jan;43(1):174-8.en
dc.identifier.issn0095-1137 (Print)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15634968-
dc.identifier.urihttp://hdl.handle.net/10371/29596-
dc.description.abstractOsteoarticular tuberculosis (OAT) is an extrapulmonary tuberculosis and accounts for 1 to 3% of all tuberculosis cases. We used an rpoB PCR-plasmid TA cloning-sequencing method to detect and identify tubercle bacilli in surgical specimens from patients suspected of having OAT. By comparing the similarities of the rpoB sequences determined with those in GenBank, Mycobacterium tuberculosis was detected in 23 of 43 samples. Three of the 23 positive samples had mutations at codon 531, which are commonly observed in rifampin-resistant M. tuberculosis strains. Our results suggest that the rpoB PCR-TA cloning-sequencing method developed, which detects M. tuberculosis and which simultaneously determines its rifampin susceptibility, can also be used efficiently for the diagnosis of OAT.en
dc.language.isoenen
dc.publisherAmerican Society for Microbiologyen
dc.subjectAntitubercular Agents/pharmacologyen
dc.subjectBiopsyen
dc.subjectCloning, Molecularen
dc.subjectDNA-Directed RNA Polymerases/*geneticsen
dc.subjectHumansen
dc.subjectJoints/*microbiologyen
dc.subjectMycobacterium tuberculosis/*classification/drugen
dc.subjecteffects/genetics/*isolation & purificationen
dc.subjectPlasmidsen
dc.subjectPolymerase Chain Reaction/*methodsen
dc.subjectRifampin/pharmacologyen
dc.subjectSequence Analysis, DNAen
dc.subjectTuberculosis, Osteoarticular/*microbiologyen
dc.titleDetection and identification of Mycobacterium tuberculosis in joint biopsy specimens by rpoB PCR cloning and sequencingen
dc.typeArticleen
dc.contributor.AlternativeAuthor윤여준-
dc.contributor.AlternativeAuthor이근화-
dc.contributor.AlternativeAuthor류윤종-
dc.contributor.AlternativeAuthor김범준-
dc.contributor.AlternativeAuthor이영호-
dc.contributor.AlternativeAuthor백구현-
dc.contributor.AlternativeAuthor김희중-
dc.contributor.AlternativeAuthor정문상-
dc.contributor.AlternativeAuthor이명철-
dc.contributor.AlternativeAuthor이상훈-
dc.contributor.AlternativeAuthor최인호-
dc.contributor.AlternativeAuthor조태준-
dc.contributor.AlternativeAuthor장봉순-
dc.contributor.AlternativeAuthor국윤호-
dc.identifier.doi10.1128/JCM.43.1.174-178.2005-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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