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ATP6V0C competes with von Hippel-Lindau protein in hypoxia-inducible factor 1alpha (HIF-1alpha) binding and mediates HIF-1alpha expression by bafilomycin A1

Cited 27 time in Web of Science Cited 26 time in Scopus
Authors
Lim, Ji-Hong; Park, Jong-Wan; Kim, Sung Joon; Kim, Myung-Suk; Park, Sang-Ki; Johnson, Randall S.; Chun, Yang-Sook
Issue Date
2006-12-21
Publisher
American Society for Pharmacology and Experimental Therapeutics (ASPET)
Citation
Mol Pharmacol. 2007 Mar;71(3):942-8. Epub 2006 Dec 18.
Keywords
Amino Acid MotifsBinding, CompetitiveEnzyme Inhibitors/*pharmacologyHumansHydrogen-Ion ConcentrationHypoxia-Inducible Factor 1, alpha Subunit/chemistry/*metabolismMacrolides/*pharmacologyVacuolar Proton-Translocating ATPases/antagonists & inhibitors/*metabolismVon Hippel-Lindau Tumor Suppressor Protein/*metabolism
Abstract
HIF-1alpha not only enables cells to survive under hypoxic conditions but also promotes cell cycle arrest and apoptosis. Therefore, its expression should be controlled at optimal levels in growing tumors. We recently reported that bafilomycin A1 exorbitantly expressed HIF-1alpha and induced the p21(WAF1/Cip1)-mediated growth arrest of tumors (Mol Pharmacol 70:1856-1865, 2006). In the present study, we addressed the mechanism underlying bafilomycin-induced HIF-1alpha expression. Bafilomycin stabilized HIF-1alpha under normoxic conditions without changes in intracellular pH. However, when ATP6V0C, the target protein of bafilomycin, was knocked down, this bafilomycin effect was significantly attenuated. Inversely, ATP6V0C expression increased HIF-1alpha levels in a gene dose-dependent manner. ATP6V0C competed with Von Hippel-Lindau protein in HIF-1alpha binding by directly interacting with HIF-1alpha, which was stimulated by bafilomycin. In confocal images, ATP6V0C was normally present in the cytoplasm but was translocated in company with HIF-1alpha to the nucleus by bafilomycin. The N-terminal end (amino acids 1-16) of HIF-1alpha was identified as the ATP6V0C-interacting motif. These results suggest that ATP6V0C, a novel regulator of HIF-1alpha, mediates HIF-1alpha expression by bafilomycin.
ISSN
0026-895X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17178925

http://hdl.handle.net/10371/29598
DOI
https://doi.org/10.1124/mol.106.030296
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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