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Chloride conductance is required for the protein kinase A and Rac1-dependent phosphorylation of moesin at Thr-558 by KCl in PC12 cells

Cited 9 time in Web of Science Cited 9 time in Scopus
Authors
Jeon, Songhee; Kim, Sohee; Kim, Eunhee; Lee, Ji Eun; Kim, Sung Joon; Juhnn, Yong-Sung; Kim, Yong Sik; Bae, Chang-Dae; Park, Joobae
Issue Date
2005-01-07
Publisher
American Society for Biochemistry and Molecular Biology
Citation
J Biol Chem. 2005 Apr 1;280(13):12181-9. Epub 2005 Jan 5.
Keywords
AnimalsChloride Channels/*metabolismChlorides/*metabolismCulture Media, Serum-Free/pharmacologyCyclic AMP-Dependent Protein Kinases/*metabolismElectrophoresis, Polyacrylamide GelImmunoblottingMicrofilament Proteins/chemistry/*metabolismPC12 CellsPatch-Clamp TechniquesPhosphorylationPlasmids/metabolismPotassium/chemistryPotassium Chloride/*metabolismRatsSignal TransductionThreonine/*metabolismTime FactorsTransfectionrac1 GTP-Binding Protein/*metabolismrhoA GTP-Binding Protein/metabolism
Abstract
Moesin is a member of the ERM family, a family of cross-linkers between the plasma membrane and the actin cytoskeleton, which are known to be activated by phosphorylation. Previously, we reported the RhoA and Rho kinase-dependent phosphorylation of moesin at Thr-558 in hippocampal neuronal cells by glutamate. Here we studied the induction of moesin phosphorylation by KCl (60 mm) in PC12 cells. Moesin phosphorylation at Thr-558 was increased after 2 min of KCl treatment, peaked at 5 min, and returned to the basal level by 60 min. KCl also activated Rac1, but not RhoA, in PC12 cells, and KCl-induced moesin phosphorylation was suppressed in dominant negative Rac1 (N17 Rac1)-expressed cells. The inhibition of protein kinase A (PKA), known as an upstream kinase of Rac1, abolished Rac1 activation and moesin phosphorylation by KCl. Interestingly, the phosphorylation of moesin by KCl was independent of KCl-induced membrane depolarization and calcium influx but was dependent on KCl-induced chloride conductance. 60 mm KCl induced chloride conductance in PC12 cells, and pretreatment with Cl- channel blocker abolished Rac1 activation and moesin phosphorylation by KCl. These results suggest that the phosphorylation of moesin at Thr-558 in PC12 cells by KCl treatment is PKA- and Rac1-dependent and that KCl-induced chloride conductance is involved in the activation of this signaling system.
ISSN
0021-9258 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15634677

http://hdl.handle.net/10371/29646
DOI
https://doi.org/10.1074/jbc.M408253200
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
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