Browse

Identification, evolution, and regulation of expression of Guinea pig trappin with an unusually long transglutaminase substrate domain

Cited 3 time in Web of Science Cited 4 time in Scopus
Authors
Furutani, Yutaka; Kato, Akira; Fibriani, Azzania; Hirata, Taku; Kawai, Ryoji; Jeon, Ju-Hong; Fujii, Yasuhisa; Kim, In-Gyu; Kojima, Soichi; Hirose, Shigehisa
Issue Date
2005-03-22
Publisher
American Society for Biochemistry and Molecular Biology
Citation
J Biol Chem. 2005 May 27;280(21):20204-15. Epub 2005 Mar 18.
Keywords
Amino Acid SequenceAndrogens/pharmacologyAnimalsBinding SitesBlotting, WesternCalcium/pharmacologyCattleCross-Linking ReagentsDNA, Complementary/chemistry*Evolution, Molecular*Gene Expression Regulation/drug effectsGuinea PigsHumansImmunohistochemistryLeukocyte Elastase/antagonists & inhibitorsMaleMiceMilk Proteins/chemistry/metabolismMolecular Sequence DataProstate/enzymologyProteins/*analysis/chemistry/*geneticsProteinase Inhibitory Proteins, SecretoryRatsSeminal Vesicles/chemistrySequence AlignmentSwineTissue DistributionTransglutaminases/*metabolism
Abstract
Trappins are found in human, bovine, hippopotamus, and members of the pig family, but not in rat and mouse. To clarify the evolution of the trappin genes and the functional significance of their products, we isolated the trappin gene in guinea pig, a species belonging to a rodent family distinct from rat and mouse. Guinea pig trappin was confirmed to encode the same domain structure as trappin, consisting of a signal sequence, an extra large transglutaminase substrate domain, and a whey acidic protein motif. Northern blot analysis and in situ hybridization histochemistry as well as immunohistochemistry demonstrated that guinea pig trappin is expressed solely in the secretory epithelium of the seminal vesicle and that its expression is androgen-dependent. We confirmed that guinea pig trappin is cross-linked by prostate transglutaminase and that the whey acidic protein motif derived from guinea pig trappin has an inhibitory activity against leukocyte elastase. Genome sequence analysis showed that guinea pig trappin belongs to the family of REST (rapidly evolving seminal vesicle transcribed) genes.
ISSN
0021-9258 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15778505

http://hdl.handle.net/10371/29647
DOI
https://doi.org/10.1074/jbc.M501678200
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse