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The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4

Cited 187 time in Web of Science Cited 202 time in Scopus
Authors
Seo, Jeong-Sun; Chong, Hyonyong; Park, Hyun Seok; Yoon, Kyoung-Oh; Jung, Cholhee; Kim, Jae Joon; Hong, Jin Han; Kim, Hyungtae; Kim, Jeong-Hyun; Kil, Joon-Il; Park, Cheol Ju; Oh, Hyun-Myung; Lee, Jung-Soon; Jin, Su-Jung; Um, Hye-Won; Lee, Hee-Jong; Oh, Soo-Jin; Kim, Jae Young; Kang, Hyung Lynn`; Lee, Se Yong; Lee, Kye Joon; Kang, Hyen Sam
Issue Date
2004-12-14
Publisher
Nature Publishing Group
Citation
Nat Biotechnol. 2005 Jan;23(1):63-8. Epub 2004 Dec 12.
Keywords
Biological TransportDNA/chemistryEthanol/chemistry/metabolismGenome*Genome, BacterialKetoglutarate Dehydrogenase Complex/metabolismMalate Dehydrogenase/metabolismModels, BiologicalMolecular Sequence DataMultigene FamilyOligonucleotide Array Sequence AnalysisOpen Reading FramesOxidative StressPlasmids/metabolismRNA/chemistrySequence Analysis, DNATranscription, GeneticTricarboxylic Acids/metabolismZymomonas/*genetics
Abstract
We report the complete genome sequence of Zymomonas mobilis ZM4 (ATCC31821), an ethanologenic microorganism of interest for the production of fuel ethanol. The genome consists of 2,056,416 base pairs forming a circular chromosome with 1,998 open reading frames (ORFs) and three ribosomal RNA transcription units. The genome lacks recognizable genes for 6-phosphofructokinase, an essential enzyme in the Embden-Meyerhof-Parnas pathway, and for two enzymes in the tricarboxylic acid cycle, the 2-oxoglutarate dehydrogenase complex and malate dehydrogenase, so glucose can be metabolized only by the Entner-Doudoroff pathway. Whole genome microarrays were used for genomic comparisons with the Z. mobilis type strain ZM1 (ATCC10988) revealing that 54 ORFs predicted to encode for transport and secretory proteins, transcriptional regulators and oxidoreductase in the ZM4 strain were absent from ZM1. Most of these ORFs were also found to be actively transcribed in association with ethanol production by ZM4.
ISSN
1087-0156 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15592456

http://hdl.handle.net/10371/29707
DOI
https://doi.org/10.1038/nbt1045
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Biochemistry & Molecular Biology (생화학교실)Journal Papers (저널논문_생화학교실)
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