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Patient-specific embryonic stem cells derived from human SCNT blastocysts

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Authors
Hwang, Woo Suk; Roh, Sung Il; Lee, Byeong Chun; Kang, Sung Keun; Kwon, Dae Kee; Kim, Sue; Kim, Sun Jong; Park, Sun Woo; Kwon, Hee Sun; Lee, Chang Kyu; Lee, Jung Bok; Kim, Jin Mee; Ahn, Curie; Paek, Sun Ha; Chang, Sang Sik; Koo, Jung Jin; Yoon, Hyun Soo; Hwang, Jung Hye; Hwang, Youn Young; Park, Ye Soo; Oh, Sun Kyung; Kim, Hee Sun; Park, Jong Hyuk; Moon, Shin Yong
Issue Date
2005-05-21
Publisher
American Association for the Advancement of Science
Citation
Science. 2005 Jun 17;308(5729):1777-83. Epub 2005 May 19.
Keywords
AdultAgammaglobulinemiaBlastocyst/*cytologyCell Differentiation*Cell LineChildChild, Preschool*Cloning, OrganismDNA FingerprintingDiabetes Mellitus, Type 1Epigenesis, GeneticEthics Committees, ResearchFemaleFibroblastsHLA Antigens/analysisHumansInformed ConsentKaryotypingMale*Nuclear Transfer TechniquesOocyte DonationPluripotent Stem Cells/*cytology/immunologySpinal Cord InjuriesStem Cell TransplantationTissue and Organ Procurement
Abstract
Patient-specific, immune-matched human embryonic stem cells (hESCs) are anticipated to be of great biomedical importance for studies of disease and development and to advance clinical deliberations regarding stem cell transplantation. Eleven hESC lines were established by somatic cell nuclear transfer (SCNT) of skin cells from patients with disease or injury into donated oocytes. These lines, nuclear transfer (NT)-hESCs, grown on human feeders from the same NT donor or from genetically unrelated individuals, were established at high rates, regardless of NT donor sex or age. NT-hESCs were pluripotent, chromosomally normal, and matched the NT patient's DNA. The major histocompatibility complex identity of each NT-hESC when compared to the patient's own showed immunological compatibility, which is important for eventual transplantation. With the generation of these NT-hESCs, evaluations of genetic and epigenetic stability can be made. Additional work remains to be done regarding the development of reliable directed differentiation and the elimination of remaining animal components. Before clinical use of these cells can occur, preclinical evidence is required to prove that transplantation of differentiated NT-hESCs can be safe, effective, and tolerated.
ISSN
1095-9203 (Electronic)
http://dx.doi.org/10.1126/science.1112286
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15905366

http://hdl.handle.net/10371/29730
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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