S-Space College of Medicine/School of Medicine (의과대학/대학원) Pediatrics (소아과학전공) Journal Papers (저널논문_소아과학전공)
Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha
- Ko, Jae-Sung; Yang, Hye-Ran; Chang, Ju-Young; Seo, Jeong-Kee
- Issue Date
- World J Gastroenterol. 2007 Apr 7;13(13):1962-5.
- Adenocarcinoma/metabolism/pathology/physiopathology; Caco-2 Cells; Colonic Neoplasms/metabolism/pathology/physiopathology; Extracellular Signal-Regulated MAP Kinases/metabolism; Humans; I-kappa B Proteins/metabolism; Interleukin-8/*metabolism; Intestinal Absorption/drug effects/*physiology; Intestinal Mucosa/drug effects/*physiology; *Lactobacillus plantarum; Membrane Potentials/drug effects/physiology; Probiotics/*pharmacology; Tumor Necrosis Factor-alpha/*pharmacology
- AIM: To determine whether Lactobacillus plantarum can modify the deleterious effects of tumor necrosis factor-alpha (TNF-alpha) on intestinal epithelial cells. METHODS: Caco-2 cells were incubated with TNF-alpha alone or in the presence of L. plantarum. Transepithelial electrical resistance was used to measure epithelial barrier function. Interleukin 8 (IL-8) secretion by intestinal epithelial cells was measured using an ELISA. Cellular lysate proteins were immunoblotted using the anti-extracellular regulated kinase (ERK), anti-phospho-ERK and anti-IkappaB-alpha. RESULTS: A TNF-alpha-induced decrease in transepithelial electrical resistance was inhibited by L. plantarum. TNF-alpha-induced IL-8 secretion was reduced by L. plantarum. L. plantarum inhibited the activation of ERK and the degradation of IkappaB-alpha in TNF-alpha-treated Caco-2 cells. CONCLUSION: Induction of epithelial barrier dysfunction and IL-8 secretion by TNF-alpha is inhibited by L. plantarum. Probiotics may preserve epithelial barrier function and inhibit the inflammatory response by altering the signal transduction pathway.
- 1007-9327 (Print)