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Lactobacillus plantarum inhibits epithelial barrier dysfunction and interleukin-8 secretion induced by tumor necrosis factor-alpha

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Authors
Ko, Jae-Sung; Yang, Hye-Ran; Chang, Ju-Young; Seo, Jeong-Kee
Issue Date
2007-04-28
Citation
World J Gastroenterol. 2007 Apr 7;13(13):1962-5.
Keywords
Adenocarcinoma/metabolism/pathology/physiopathologyCaco-2 CellsColonic Neoplasms/metabolism/pathology/physiopathologyExtracellular Signal-Regulated MAP Kinases/metabolismHumansI-kappa B Proteins/metabolismInterleukin-8/*metabolismIntestinal Absorption/drug effects/*physiologyIntestinal Mucosa/drug effects/*physiology*Lactobacillus plantarumMembrane Potentials/drug effects/physiologyProbiotics/*pharmacologyTumor Necrosis Factor-alpha/*pharmacology
Abstract
AIM: To determine whether Lactobacillus plantarum can modify the deleterious effects of tumor necrosis factor-alpha (TNF-alpha) on intestinal epithelial cells. METHODS: Caco-2 cells were incubated with TNF-alpha alone or in the presence of L. plantarum. Transepithelial electrical resistance was used to measure epithelial barrier function. Interleukin 8 (IL-8) secretion by intestinal epithelial cells was measured using an ELISA. Cellular lysate proteins were immunoblotted using the anti-extracellular regulated kinase (ERK), anti-phospho-ERK and anti-IkappaB-alpha. RESULTS: A TNF-alpha-induced decrease in transepithelial electrical resistance was inhibited by L. plantarum. TNF-alpha-induced IL-8 secretion was reduced by L. plantarum. L. plantarum inhibited the activation of ERK and the degradation of IkappaB-alpha in TNF-alpha-treated Caco-2 cells. CONCLUSION: Induction of epithelial barrier dysfunction and IL-8 secretion by TNF-alpha is inhibited by L. plantarum. Probiotics may preserve epithelial barrier function and inhibit the inflammatory response by altering the signal transduction pathway.
ISSN
1007-9327 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17461497

http://hdl.handle.net/10371/30201
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College of Medicine/School of Medicine (의과대학/대학원)Pediatrics (소아과학전공)Journal Papers (저널논문_소아과학전공)
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