Browse
S-Space
College of Medicine/School of Medicine (의과대학/대학원)
Pathology (병리학전공)
Journal Papers (저널논문_병리학전공)
Ongoing debates in the assessment of the HER2 status in breast cancer: A comparative analysis by immunohistochemistry and FISH using original FDA and ASCO/CAP guidelines
- Issue Date
- 2008-10-22
- Publisher
- Blackwell Publishing
- Citation
- Bas Appl Pathol 2008; 1: 182-188
- Keywords
- ASCO/CAP ; breast carcinoma ; FISH ; HER2 ; immunohistochemistry
- Description
- The definitive version is available at www.blackwell-synergy.com.
- Abstract
- Background and aims: HER2 status should be precisely evaluated in breast cancer to ensure appropriate patient management. Method: We evaluated HER2 status in 755 breast cancers including 92 consecutively resected cases by immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH). Results: For the 92 consecutive cases, the positive rate of HER2 IHC (2+ and 3+) was 25.0%, and HER2 amplification (HER2/CEP17 ratio 2265 2.0) was observed in 21.7%. Of the 755 cases, 88% of IHC 3+ cases; 57% of IHC 2+ cases; 15% of IHC 1+ cases; and 1% of IHC 0 cases showed HER2 amplification. Notably, 20 (2.6%) of the 755 cases exhibited a HER2/CEP17 ratio between 1.8 and 2.2, which was recently classified equivocal by the ASCO/CAP. Most of them revealed HER2 high polysomy with an average copy number per cell between 3.29 and 7.24. Immunohistochemically, 4 of the 20 cases were luminal A, 12 were luminal B, and 3 were HER2 subtype. Conclusions: The relatively high rate of HER2 amplification among IHC 1+ or 2+ cases at our institute emphasizes the importance of validating HER2 testing in different laboratories and the need for complementary HER2 FISH in IHC 1+ patients. Most patients equivocal for HER2 amplification had HER2 high polysomy and expressed hormone receptor.
- ISSN
- 1755-9294
- Language
- English
- Files in This Item: There are no files associated with this item.
- Appears in Collections:
- College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.