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Involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity

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Authors
Kim, Byung Joo; Jun, Jae Yeol; So, Insuk; Kim, Ki Whan
Issue Date
2006-03-08
Publisher
WJG Press
Citation
World J Gastroenterol. 2006 Feb 7;12(5):796-9.
Keywords
AnimalsBiological Clocks/drug effects/*physiologyClonazepam/analogs & derivatives/pharmacologyFemaleIntestine, Small/cytology/drug effects/*metabolismMaleMiceMice, Inbred BALB CMitochondria/drug effects/metabolismNickel/pharmacologyPatch-Clamp TechniquesSodium-Calcium Exchanger/antagonists & inhibitors/*metabolismThiazepines/pharmacology
Abstract
AIM: Interstitial cells of Cajal (ICCs) are the pacemaker cells that generate slow waves in the gastrointestinal (GI) tract. We have aimed to investigate the involvement of mitochondrial Na+-Ca2+ exchange in intestinal pacemaking activity in cultured interstitial cells of Cajal. METHODS: Enzymatic digestions were used to dissociate ICCs from the small intestine of a mouse. The whole-cell patch-clamp configuration was used to record membrane currents (voltage clamp) and potentials (current clamp) from cultured ICCs. RESULTS: Clonazepam and CGP37157 inhibited the pacemaking activity of ICCs in a dose-dependent manner. Clonazepam from 20 to 60 micromol/L and CGP37157 from 10 to 30 micromol/L effectively inhibited Ca2+ efflux from mitochondria in pacemaking activity of ICCs. The IC50s of clonazepam and CGP37157 were 37.1 and 18.2 micromol/L, respectively. The addition of 20 micromol/L NiCl2 to the internal solution caused a "wax and wane" phenomenon of pacemaking activity of ICCs. CONCLUSION: These results suggest that mitochondrial Na+-Ca2+ exchange has an important role in intestinal pacemaking activity.
ISSN
1007-9327 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16521198

http://hdl.handle.net/10371/36239
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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