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Rapid and simple identification of Orientia tsutsugamushi from other group rickettsiae by duplex PCR assay using groEL gene

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Authors
Park, Hyo-Soon; Lee, Jung-Hee; Jeong, Eun-Ju; Kim, Jung-Eun; Hong, Sung-Jin; Park, Tae-Kyu; Kim, Tae-Yung; Jang, Won-Jong; Park, Kyung-Hee; Kim, Bum-Joon; Kook, Yoon-Hoh; Lee, Seung-Hyun
Issue Date
2005-06-21
Publisher
Wiley-Blackwell
Citation
Microbiol Immunol. 2005;49(6):545-9.
Keywords
*Bacterial Typing TechniquesDNA, Ribosomal/analysis/geneticsGroEL Protein/*geneticsOrientia tsutsugamushi/genetics/immunology/*isolation &purification/pathogenicityPolymerase Chain ReactionScrub Typhus/*epidemiology/immunology*Sequence Analysis, DNATyphus, Endemic Flea-Borne/*epidemiology/immunology
Abstract
In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229-bp and the 366-bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397-bp and the 213-bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.
ISSN
0385-5600 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15965302

http://hdl.handle.net/10371/36676
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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