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p66Shc expression in proliferating thyroid cells is regulated by thyrotropin receptor signaling

Cited 22 time in Web of Science Cited 23 time in Scopus
Authors
Park, Y J; Kim, T Y; Lee, S H; Kim, H; Kim, S W; Shong, M; Yoon, Y K; Cho, B Y; Park, D J
Issue Date
2005-02-12
Publisher
Endocrine Society
Citation
Endocrinology. 2005 May;146(5):2473-80. Epub 2005 Feb 10.
Keywords
Adaptor Proteins, Signal Transducing/*geneticsAdultAgedAnimalsAutoantibodies/bloodCell Division/drug effectsCell LineCells, CulturedCyclic AMP/pharmacologyCyclic AMP-Dependent Protein Kinases/pharmacologyFemaleGTP-Binding Protein alpha Subunits, Gs/physiologyGene Expression Regulation/drug effects/*physiologyGoiter/metabolismHumansInsulin-Like Growth Factor I/pharmacologyMaleMiddle AgedRNA, Messenger/analysisRatsRats, Sprague-DawleyReceptors, Thyrotropin/immunology/*physiologyShc Signaling Adaptor Proteins*Signal TransductionThyroid Gland/chemistry/cytology/*metabolismThyrotropin/pharmacology
Abstract
It is almost unanimously accepted that thyrocyte proliferation is synergistically activated by TSH and insulin/IGF-I. Moreover, it was recently suggested that p66Shc, which is an adaptor molecule of the IGF-I receptor, might play a critical role in this synergistic effect. In this study, we undertook to confirm the role and the mechanism underlying the regulation of p66Shc expression via TSH receptor in thyrocytes. We have found that p66Shc expression is elevated in proliferating human thyroid tissues, including adenomatous goiter, adenoma, Graves' disease, and thyroid cancer, but not in normal thyroid. Among growth factors, TSH increased p66Shc expression both in vivo and in vitro; however, IGF-I, epidermal growth factor, or insulin did not. TSH and Graves' Ig increased the p66Shc expression via the TSH receptor-G(s)-cAMP pathway. However, interestingly, IGF-I or epidermal growth factor increased the tyrosine phosphorylations of p66Shc, and this was enhanced by TSH pretreatment. A similar synergism was observed during the DNA synthesis. When we measured the p66Shc levels induced by individual Igs from 130 patients with Graves' disease, TSH receptor stimulating activity and goiter size showed a weak correlation. We conclude that the expression of p66Shc is regulated by signaling through the TSH receptor in proliferating thyroid cells and that p66Shc appears to be an important mediator of the synergistic effect between TSH and IGF-I with respect to thyrocyte proliferation. Moreover, we suggest that TSH potentiates the regulatory effect of IGF-I on thyrocyte growth, at least in part, by increasing the expression of p66Shc.
ISSN
0013-7227 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15705774

http://hdl.handle.net/10371/36678
DOI
https://doi.org/10.1210/en.2004-1588
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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