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Indole-3-acetic acid/horseradish peroxidase-induced apoptosis involves cell surface CD95 (Fas/APO-1) expression

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Authors
Kim, Dong-Seok; Kim, So-Young; Jeong, Yun-Mi; Jeon, Sang-Eun; Kim, Myo-Kyoung; Kwon, Sun-Bang; Park, Kyoung-Chan
Issue Date
2006-08-02
Publisher
Pharmaceutical Society of Japan
Citation
Biol Pharm Bull. 2006 Aug;29(8):1625-9.
Keywords
Antibodies/immunologyAntigens, CD95/immunology/*metabolismApoptosis/*drug effectsBlotting, WesternCatalase/metabolismCell Line, TumorCell Membrane/metabolismFlow CytometryHorseradish Peroxidase/*pharmacologyHumansIndoleacetic Acids/*pharmacologyMicroscopy, Confocal
Abstract
Recently, we showed that a combination of indole-3-acetic acid (IAA) and horseradish peroxidase (HRP) produces hydrogen peroxide (H2O2), and that this leads to the apoptosis of G361 human melanoma cells. In the present study, flow cytometric analysis confirmed that H2O2 is involved the IAA/HRP-induced apoptotic process. We also found that IAA/HRP increases cell surface CD95 (Fas/APO-1) expression, and that this is blocked by catalase treatment. Furthermore, blocking CD95 with a neutralizing antibody significantly restored IAA/HRP-induced apoptosis. In addition, the IAA/HRP-induced activations of CD95 downstream molecules, i.e., caspase-8, Bid, and caspase-3, were also inhibited by catalase. Moreover, a caspase-8 inhibitor significantly blocked IAA/HRP-induced apoptosis. These results indicate that IAA/HRP-induced apoptosis involves a CD95-initiated death receptor signaling pathway initiated by hydrogen peroxide.
ISSN
0918-6158 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=16880616

http://hdl.handle.net/10371/39190
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College of Medicine/School of Medicine (의과대학/대학원)Molecular and Genomic Medicine (분자유전체의학전공)Journal Papers (저널논문_분자유전체의학전공)
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