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Semiquantitative Measurement of Murine Bleomycin-Induced Lung Fibrosis in In Vivo and Postmortem Conditions Using Microcomputed Tomography: Correlation With Pathologic Scores--Initial Results
DC Field | Value | Language |
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dc.contributor.author | Lee, Hyun Ju | - |
dc.contributor.author | Goo, Jin Mo | - |
dc.contributor.author | Kim, Na Ra | - |
dc.contributor.author | Kim, Min A | - |
dc.contributor.author | Chung, Doo Hyun | - |
dc.contributor.author | Son, Kyu-Ri | - |
dc.contributor.author | Kim, Hyo-Cheol | - |
dc.contributor.author | Lee, Chang Hyun | - |
dc.contributor.author | Park, Chang Min | - |
dc.contributor.author | Chun, Eun Ju | - |
dc.contributor.author | Im, Jung-Gi | - |
dc.date.accessioned | 2009-06-01T22:52:31Z | - |
dc.date.available | 2009-06-01T22:52:31Z | - |
dc.date.issued | 2008-05-23 | - |
dc.identifier.citation | Invest Radiol. 2008;43:453-460 | en |
dc.identifier.issn | 0020-9996 (print) | - |
dc.identifier.issn | 1536-0210 (online) | - |
dc.identifier.uri | https://hdl.handle.net/10371/4232 | - |
dc.description.abstract | To evaluate whether the semiquantification of lung inflammation and fibrosis in murine bleomycin-induced lung fibrosis using micro-computed tomography (micro-CT) in in vivo and postmortem conditions is feasible, and to correlate micro-CT and pathologic scores. MATERIALS AND METHODS: Bleomycin-induced lung fibrosis was created by intratracheally instilling 3 mg/kg of bleomycin into C57BL/6 mice. Mice were allocated randomly to 2-week, 4-week, and 8-week follow-up groups. In each group, in vivo and follow-up postmortem micro-CT were performed using a voxel size of 35 x 35 x 35 microm. Ground-glass opacity (GGO), consolidation, parenchymal lines, honeycombing, and peripheral bronchial dilatation were scored on micro-CT images in a semiquantitative fashion, whereas inflammation and fibrosis were scored histopathologically. The confidence levels of micro-CT findings were also scored. Correlations between micro-CT and pathologic findings were examined using Spearman rank correlation analysis, and differences between CT scores and confidence levels for in vivo and postmortem micro-CT were subjected to Wilcoxon signed rank testing. Agreements between in vivo and postmortem micro-CT scores were tested using weighted kappa statistics. RESULTS: Consolidation in vivo (r = 0.46) and at postmortem (r = 0.39) and GGO in vivo (r = 0.31) by micro-CT showed fair to moderate correlation with pathologic inflammation scores (P < 0.001). By in vivo and postmortem micro-CT, parenchymal lines (r = 0.72 vs. 0.83) showed good to excellent and peripheral bronchial dilatation (r = 0.47 vs. 0.68) showed moderate to good correlation with pathologic fibrosis scores (P < 0.001). For GGO, consolidation, peripheral bronchial dilatation, and parenchymal lines, fair to moderate agreement was obtained between in vivo and postmortem micro-CT. However, confidence levels for peripheral bronchial dilatation, parenchymal lines, and honeycombing were significantly higher by postmortem micro-CT (P < 0.001). CONCLUSIONS: Micro-CT scores and pathologic scores were found to be well correlated by in vivo and postmortem micro-CT. Although agreements between in vivo and postmortem micro-CT were significant, the confidence levels for fibrosis-related CT findings were significantly higher by postmortem micro-CT. | en |
dc.description.sponsorship | Supported by Seoul National University Hospital Research Grant (04-2005-014-0). | en |
dc.language.iso | en | - |
dc.publisher | Lippincott, Williams & Wilkins | en |
dc.subject | microcomputed tomography | en |
dc.subject | bleomycin, fibrosis | en |
dc.subject | lung | en |
dc.subject | murine | en |
dc.title | Semiquantitative Measurement of Murine Bleomycin-Induced Lung Fibrosis in In Vivo and Postmortem Conditions Using Microcomputed Tomography: Correlation With Pathologic Scores--Initial Results | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이현주 | - |
dc.contributor.AlternativeAuthor | 구진모 | - |
dc.contributor.AlternativeAuthor | 김나라 | - |
dc.contributor.AlternativeAuthor | 김민아 | - |
dc.contributor.AlternativeAuthor | 정두현 | - |
dc.contributor.AlternativeAuthor | 손규리 | - |
dc.contributor.AlternativeAuthor | 김효철 | - |
dc.contributor.AlternativeAuthor | 이창현 | - |
dc.contributor.AlternativeAuthor | 박창민 | - |
dc.contributor.AlternativeAuthor | 전은주 | - |
dc.contributor.AlternativeAuthor | 임정기 | - |
dc.identifier.doi | 10.1097/RLI.0b013e31816900ec | - |
dc.identifier.doi | 10.1097/RLI.0b013e31816900ec | - |
dc.citation.journaltitle | Investigative Radiology | - |
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