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Semiquantitative Measurement of Murine Bleomycin-Induced Lung Fibrosis in In Vivo and Postmortem Conditions Using Microcomputed Tomography: Correlation With Pathologic Scores--Initial Results

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dc.contributor.authorLee, Hyun Ju-
dc.contributor.authorGoo, Jin Mo-
dc.contributor.authorKim, Na Ra-
dc.contributor.authorKim, Min A-
dc.contributor.authorChung, Doo Hyun-
dc.contributor.authorSon, Kyu-Ri-
dc.contributor.authorKim, Hyo-Cheol-
dc.contributor.authorLee, Chang Hyun-
dc.contributor.authorPark, Chang Min-
dc.contributor.authorChun, Eun Ju-
dc.contributor.authorIm, Jung-Gi-
dc.date.accessioned2009-06-01T22:52:31Z-
dc.date.available2009-06-01T22:52:31Z-
dc.date.issued2008-05-23-
dc.identifier.citationInvest Radiol. 2008;43:453-460en
dc.identifier.issn0020-9996 (print)-
dc.identifier.issn1536-0210 (online)-
dc.identifier.urihttp://hdl.handle.net/10371/4232-
dc.description.abstractTo evaluate whether the semiquantification of lung inflammation and fibrosis in murine bleomycin-induced lung fibrosis using micro-computed tomography (micro-CT) in in vivo and postmortem conditions is feasible, and to correlate micro-CT and pathologic scores. MATERIALS AND METHODS: Bleomycin-induced lung fibrosis was created by intratracheally instilling 3 mg/kg of bleomycin into C57BL/6 mice. Mice were allocated randomly to 2-week, 4-week, and 8-week follow-up groups. In each group, in vivo and follow-up postmortem micro-CT were performed using a voxel size of 35 x 35 x 35 microm. Ground-glass opacity (GGO), consolidation, parenchymal lines, honeycombing, and peripheral bronchial dilatation were scored on micro-CT images in a semiquantitative fashion, whereas inflammation and fibrosis were scored histopathologically. The confidence levels of micro-CT findings were also scored. Correlations between micro-CT and pathologic findings were examined using Spearman rank correlation analysis, and differences between CT scores and confidence levels for in vivo and postmortem micro-CT were subjected to Wilcoxon signed rank testing. Agreements between in vivo and postmortem micro-CT scores were tested using weighted kappa statistics. RESULTS: Consolidation in vivo (r = 0.46) and at postmortem (r = 0.39) and GGO in vivo (r = 0.31) by micro-CT showed fair to moderate correlation with pathologic inflammation scores (P < 0.001). By in vivo and postmortem micro-CT, parenchymal lines (r = 0.72 vs. 0.83) showed good to excellent and peripheral bronchial dilatation (r = 0.47 vs. 0.68) showed moderate to good correlation with pathologic fibrosis scores (P < 0.001). For GGO, consolidation, peripheral bronchial dilatation, and parenchymal lines, fair to moderate agreement was obtained between in vivo and postmortem micro-CT. However, confidence levels for peripheral bronchial dilatation, parenchymal lines, and honeycombing were significantly higher by postmortem micro-CT (P < 0.001). CONCLUSIONS: Micro-CT scores and pathologic scores were found to be well correlated by in vivo and postmortem micro-CT. Although agreements between in vivo and postmortem micro-CT were significant, the confidence levels for fibrosis-related CT findings were significantly higher by postmortem micro-CT.en
dc.description.sponsorshipSupported by Seoul National University Hospital Research Grant (04-2005-014-0).en
dc.language.isoen-
dc.publisherLippincott, Williams & Wilkinsen
dc.subjectmicrocomputed tomographyen
dc.subjectbleomycin, fibrosisen
dc.subjectlungen
dc.subjectmurineen
dc.titleSemiquantitative Measurement of Murine Bleomycin-Induced Lung Fibrosis in In Vivo and Postmortem Conditions Using Microcomputed Tomography: Correlation With Pathologic Scores--Initial Resultsen
dc.typeArticleen
dc.contributor.AlternativeAuthor이현주-
dc.contributor.AlternativeAuthor구진모-
dc.contributor.AlternativeAuthor김나라-
dc.contributor.AlternativeAuthor김민아-
dc.contributor.AlternativeAuthor정두현-
dc.contributor.AlternativeAuthor손규리-
dc.contributor.AlternativeAuthor김효철-
dc.contributor.AlternativeAuthor이창현-
dc.contributor.AlternativeAuthor박창민-
dc.contributor.AlternativeAuthor전은주-
dc.contributor.AlternativeAuthor임정기-
dc.identifier.doi10.1097/RLI.0b013e31816900ec-
dc.identifier.doi10.1097/RLI.0b013e31816900ec-
dc.citation.journaltitleInvestigative Radiology-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Pathology (병리학전공)Journal Papers (저널논문_병리학전공)
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