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Diagnostic usefulness of a T-cell-based assay for extrapulmonary tuberculosis in immunocompromised patients
Cited 66 time in
Web of Science
Cited 73 time in Scopus
- Authors
- Issue Date
- 2009-02-03
- Publisher
- Elsevier
- Citation
- Am J Med. 2009 Feb;122(2):189-95.
- Keywords
- Adult ; Antigens, Bacterial/immunology ; Bacterial Proteins/immunology ; Female ; Humans ; Interferon-gamma/metabolism ; Male ; Middle Aged ; Predictive Value of Tests ; Sensitivity and Specificity ; T-Lymphocytes/*immunology ; Tuberculin Test ; Tuberculosis/*diagnosis/immunology ; Enzyme-Linked Immunosorbent Assay ; Immunocompromised Host
- Abstract
- BACKGROUND: The low reactivity of the tuberculin skin test limits its clinical use in immunocompromised patients with extrapulmonary tuberculosis. A recently developed T-cell-based assay for diagnosing tuberculosis infection gave promising results. However, there were few data on the usefulness of this assay for diagnosing extrapulmonary tuberculosis in immunocompromised patients. METHODS: All adult patients with suspected extrapulmonary tuberculosis were prospectively enrolled at 2 university-affiliated hospitals over an 18-month period. In addition to the conventional tests for diagnosing extrapulmonary tuberculosis, enzyme-linked immunospot (ELISPOT) assay for the interferon-gamma-producing T-cell response to early secretory antigenic target-6 and culture filtrate protein-10 was performed. The final diagnoses in patients with suspected extrapulmonary tuberculosis were classified by clinical category. RESULTS: There were 179 patients with suspected extrapulmonary tuberculosis enrolled: 59 (33%) were classified as immunocompromised. Of the 179 patients, 75 (42%) were classified as extrapulmonary tuberculosis, including 56 confirmed tuberculosis plus 19 probable tuberculosis, and 97 (54%) were classified as not tuberculosis. The remaining 7 (4%) had possible tuberculosis and were excluded from the final analysis. The tuberculin skin test (induration size >or=10 mm) was less sensitive in immunocompromised patients (38%; 95% confidence interval [CI], 19%-59%) than in immunocompetent patients (69%; 95% CI, 54%-81%, P=.01). In contrast, the ELISPOT assay retained a high sensitivity: (88%; 95% CI, 68%-97%) in immunocompromised patients compared with 96% (95% CI, 87%-100%) in immunocompetent patients (P=.32). CONCLUSION: The immunosuppressive condition does not affect the diagnostic sensitivity of the ELISPOT assay for extrapulmonary tuberculosis.
- ISSN
- 1555-7162 (Electronic)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19185094
http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6TDC-4VG98GH-J-1&_cdi=5195&_user=168665&_orig=search&_coverDate=02%2F28%2F2009&_sk=998779997&view=c&wchp=dGLzVlz-zSkWz&md5=c30c37ae87947c9a197058e6f70b11f9&ie=/sdarticle.pdf
https://hdl.handle.net/10371/45949
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