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Decreased phosphorylation of STAT-1, STAT-4 and cytokine release in MDR-TB patients with primary resistance
DC Field | Value | Language |
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dc.contributor.author | Kim, H. J. | - |
dc.contributor.author | Chung, D. H. | - |
dc.contributor.author | Kim, M. J, | - |
dc.contributor.author | Jang, J. H. | - |
dc.contributor.author | Kim, Y. W. | - |
dc.contributor.author | Han, S. K. | - |
dc.contributor.author | Shim, Y-W. | - |
dc.contributor.author | Yim, J-J. | - |
dc.date.accessioned | 2009-06-12T08:28:26Z | - |
dc.date.available | 2009-06-12T08:28:26Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Int J Tuberc Lung Dis 2008; 12: 1071-1076 | en |
dc.identifier.issn | 1027-3719 (print) | - |
dc.identifier.issn | 1815-7920 (online) | - |
dc.identifier.uri | http://www.ingentaconnect.com/content/iuatld/ijtld/2008/00000012/00000009/art00016 | - |
dc.identifier.uri | https://hdl.handle.net/10371/4615 | - |
dc.description.abstract | SETTING: We recently showed that treatment failure rate was higher among multidrug-resistant tuberculosis (MDR-TB) patients without a previous history of tuberculosis (TB) treatment, or so-called `primary resistance'.
OBJECTIVE: To investigate the phosphorylation levels of signal transducers and activators of transcription-1 (STAT-1) and STAT-4 and the subsequent cytokine release as a possible cause of a poor prognosis in MDR-TB patients with primary resistance. DESIGN: Ten patients with successfully treated pulmonary TB without resistance, 12 MDR-TB patients with acquired resistance and 10 MDR-TB patients with primary resistance were enrolled. After 24 h stimulation of peripheral blood mononuclear cells (PBMC) with interferon-gamma (IFN-γ), interleukin-12 (IL-12), purified protein derivative (PPD), or lysate of Mycobacterium tuberculosis, flow cytometric analysis of intracellular pSTAT-1 and pSTAT-4 were performed and secretion of IFN-γ, IL-12p40 and tumour necrosis factor-alpha (TNF-α) was measured in culture supernatant. RESULTS: The mean fluorescent intensities of pSTAT-1 and pSTAT-4 in PBMC of MDR-TB patients with primary resistance decreased on stimulation of IFN-γ, PPD or lysate of M. tuberculosis when compared with patients with acquired resistance. In addition, secretion of IFN-γ, IL-12p40 and TNF-α in these patients decreased on various stimuli. CONCLUSION: Decreased phosphorylation of STAT-1, STAT-4, and of subsequent cytokine release, might be associated with a poor prognosis in MDR-TB patients with primary resistance. | en |
dc.language.iso | en | - |
dc.publisher | International Union Against Tuberculosis and Lung Disease | en |
dc.subject | MDR-TB | en |
dc.subject | IFN-γ | en |
dc.subject | IL-12 | en |
dc.subject | TNF-α | en |
dc.subject | pSTAT-4 | en |
dc.subject | pSTAT-1 | en |
dc.title | Decreased phosphorylation of STAT-1, STAT-4 and cytokine release in MDR-TB patients with primary resistance | en |
dc.type | Article | en |
dc.citation.journaltitle | The international journal of tuberculosis and lung disease | - |
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