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Association of CD47 with natural killer cell-mediated cytotoxicity of head-and-neck squamous cell carcinoma lines

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Authors
Kim, Min Jung; Lee, June-Chul; Lee, Jae-Jung; Kim, Soyeon; Lee, Sang Goo; Park, Seok-Woo; Sung, Myung Whun; Heo, Dae Seog
Issue Date
2008-05-24
Publisher
Karger
Citation
Tumour Biol. 2008;29(1):28-34. Epub 2008 May 23.
Keywords
Antigens, CD47/genetics/*immunologyCarcinoma, Squamous Cell/*immunologyCell Line, TumorCytotoxicity, ImmunologicHead and Neck Neoplasms/*immunologyHumansKiller Cells, Natural/*immunologyTransfection
Abstract
OBJECTIVE: Integrin-associated protein (CD47) binds specifically to the inhibitory receptor signal-regulatory protein. This study was designed to evaluate the role of CD47 in natural killer (NK) cell-mediated cytotoxicity against cancer cells. METHODS: Head-and-neck squamous cell carcinoma (HNSCC) cell lines were analyzed for the expression of CD47 and susceptibility to NK cell-mediated killing. Cytolytic activity was assessed by (51)Cr-specific release assays and by measuring cytokine production. RESULTS: HNSCC cell lines that had high CD47 expression showed lower levels of NK cytotoxicity than those with low CD47 expression. After pre-treating cells with neutralizing major histocompatibility complex (MHC) class I or anti-CD47 antibodies, NK cell-mediated cytotoxicity against HNSCC cell lines increased. In addition, when CD47 cDNA was transfected into Caco-2 cells, NK cell-mediated cytotoxicity decreased. CONCLUSION: These findings suggest that CD47 may play an inhibitory role in NK cell-mediated cytotoxicity against cancer cells, implying a possible mechanism of immune escape in human cancer.
ISSN
1423-0380 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18497546

http://content.karger.com/ProdukteDB/produkte.asp?Aktion=ShowPDF&ArtikelNr=000132568&Ausgabe=237395&ProduktNr=224124&filename=000132568.pdf

http://hdl.handle.net/10371/46311
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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