S-Space College of Medicine/School of Medicine (의과대학/대학원) Internal Medicine (내과학전공) Journal Papers (저널논문_내과학전공)
Association of CD47 with natural killer cell-mediated cytotoxicity of head-and-neck squamous cell carcinoma lines
- Kim, Min Jung; Lee, June-Chul; Lee, Jae-Jung; Kim, Soyeon; Lee, Sang Goo; Park, Seok-Woo; Sung, Myung Whun; Heo, Dae Seog
- Issue Date
- Tumour Biol. 2008;29(1):28-34. Epub 2008 May 23.
- Antigens, CD47/genetics/*immunology; Carcinoma, Squamous Cell/*immunology; Cell Line, Tumor; Cytotoxicity, Immunologic; Head and Neck Neoplasms/*immunology; Humans; Killer Cells, Natural/*immunology; Transfection
- OBJECTIVE: Integrin-associated protein (CD47) binds specifically to the inhibitory receptor signal-regulatory protein. This study was designed to evaluate the role of CD47 in natural killer (NK) cell-mediated cytotoxicity against cancer cells. METHODS: Head-and-neck squamous cell carcinoma (HNSCC) cell lines were analyzed for the expression of CD47 and susceptibility to NK cell-mediated killing. Cytolytic activity was assessed by (51)Cr-specific release assays and by measuring cytokine production. RESULTS: HNSCC cell lines that had high CD47 expression showed lower levels of NK cytotoxicity than those with low CD47 expression. After pre-treating cells with neutralizing major histocompatibility complex (MHC) class I or anti-CD47 antibodies, NK cell-mediated cytotoxicity against HNSCC cell lines increased. In addition, when CD47 cDNA was transfected into Caco-2 cells, NK cell-mediated cytotoxicity decreased. CONCLUSION: These findings suggest that CD47 may play an inhibitory role in NK cell-mediated cytotoxicity against cancer cells, implying a possible mechanism of immune escape in human cancer.
- 1423-0380 (Electronic)
- Files in This Item: There are no files associated with this item.