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TGF-beta1-mediated activations of c-Src and Rac1 modulate levels of cyclins and p27(Kip1) CDK inhibitor in hepatoma cells replated on fibronectin
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hwang-Phill | - |
dc.contributor.author | Kim, Tai-Young | - |
dc.contributor.author | Lee, Mi-Sook | - |
dc.contributor.author | Jong, Hyun-Soon | - |
dc.contributor.author | Kim, Tae-You | - |
dc.contributor.author | Lee, Jung Weon | - |
dc.contributor.author | Bang, Yung-Jue | - |
dc.date.accessioned | 2010-01-29T15:20:39Z | - |
dc.date.available | 2010-01-29T15:20:39Z | - |
dc.date.issued | 2005-03-22 | - |
dc.identifier.citation | Biochim Biophys Acta. 2005 Mar 22;1743(1-2):151-61. | en |
dc.identifier.issn | 0006-3002 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=15777850 | - |
dc.identifier.uri | https://hdl.handle.net/10371/47148 | - |
dc.description.abstract | Integrin-mediated cell adhesion transduces signals to regulate actin cytoskeleton and cell proliferation. While understanding how integrin signals cross-talk with the TGF-beta1 pathways, we observed lamellipodia formation and cyclin regulation in Hep3B cells, following TGF-beta1 treatment. To answer if integrin signaling via actin organization might regulate cell cycle progression after TGF-beta1 treatment, we analyzed cross-talk between the two receptor-mediated pathways in hepatoma cells on specific ECMs. We found that basal and TGF-beta1-mediated activation of c-Src and Rac1, expression of cyclins E and A, and suppression of p27Kip1 were significant in cells replated on fibronectin, but not in cells on collagen I, indicating a different integrin-mediated cellular response to TGF-beta1 treatment. Levels of tyrosine phosphorylation and actin-enriched lamellipodia on fibronectin were also more prominent than in cells on collagen I. Studies using pharmacological inhibitors or transient transfections revealed that the preferential TGF-beta1 effects in cells on fibronectin required c-Src family kinase activity. These observations suggest that a specific cross-talk between TGF-beta1 and fibronectin-binding integrin signal pathways leads to the activation of c-Src/Rac1/actin-organization, leading to changes in cell cycle regulator levels in hepatoma cells. Therefore, this study represents another mechanism to regulate cell cycle regulators when integrin signaling is collaborative with TGF-beta1 pathways. | en |
dc.language.iso | en | en |
dc.publisher | Elsevier | en |
dc.subject | Actins/metabolism | en |
dc.subject | Blotting, Western | en |
dc.subject | Bromodeoxyuridine/pharmacology | en |
dc.subject | Carcinoma, Hepatocellular/*metabolism | en |
dc.subject | Cell Adhesion | en |
dc.subject | Cell Cycle | en |
dc.subject | Cell Cycle Proteins/*metabolism | en |
dc.subject | Cell Line | en |
dc.subject | Cell Line, Tumor | en |
dc.subject | Cell Proliferation | en |
dc.subject | Collagen Type I/metabolism | en |
dc.subject | Cyclin A/metabolism | en |
dc.subject | Cyclin E/metabolism | en |
dc.subject | Cyclin-Dependent Kinase Inhibitor p27 | en |
dc.subject | Cytoskeleton | en |
dc.subject | Electrophoresis, Polyacrylamide Gel | en |
dc.subject | Fibronectins/*metabolism | en |
dc.subject | Flow Cytometry | en |
dc.subject | Fluorescent Antibody Technique, Indirect | en |
dc.subject | Gene Expression Regulation | en |
dc.subject | Humans | en |
dc.subject | Integrin alpha2/metabolism | en |
dc.subject | Integrins/metabolism | en |
dc.subject | Microscopy, Phase-Contrast | en |
dc.subject | Mutation | en |
dc.subject | Phosphotransferases/*metabolism | en |
dc.subject | Protein Binding | en |
dc.subject | Protein-Tyrosine Kinases | en |
dc.subject | Proto-Oncogene Proteins/*metabolism | en |
dc.subject | Signal Transduction | en |
dc.subject | Transfection | en |
dc.subject | Transforming Growth Factor beta/*metabolism | en |
dc.subject | Transforming Growth Factor beta1 | en |
dc.subject | Tumor Suppressor Proteins/*metabolism | en |
dc.subject | rac1 GTP-Binding Protein/*metabolism | en |
dc.title | TGF-beta1-mediated activations of c-Src and Rac1 modulate levels of cyclins and p27(Kip1) CDK inhibitor in hepatoma cells replated on fibronectin | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김황필 | - |
dc.contributor.AlternativeAuthor | 김태영 | - |
dc.contributor.AlternativeAuthor | 이미숙 | - |
dc.contributor.AlternativeAuthor | 종현순 | - |
dc.contributor.AlternativeAuthor | 김태유 | - |
dc.contributor.AlternativeAuthor | 이정원 | - |
dc.contributor.AlternativeAuthor | 방영주 | - |
dc.identifier.doi | 10.1016/j.bbamcr.2004.09.014 | - |
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