S-Space College of Dentistry/School of Dentistry (치과대학/치의학대학원) Dept. of Dentistry (치의학과) Journal Papers (저널논문_치의학과)
Effects of sonicated Enterrococcus faecalis extracts on Interleukin-2 and Interleukin-4 production by human T cells
- Son, Ho-Hyun; Lim, SungSam; Shon, WonJun; Kim, Hyeon-Sik; Lee, WooCheol
- Issue Date
- JOURNAL OF ENDODONTICS 2004;30:701-703.
- In the present authors’ previous study, sonicated
Enterococcus faecalis extracts were shown to
suppress the cell cycle progression of human lymphocytes.
To study the effect of this microorganism
on the function of lymphocytes, the authors
investigated the levels of interleukin-2 (IL-2) and
interleukin-4 (IL-4) production by T lymphocytes
before and after the addition of sonicated E. faecalis
extracts. In this study, levels of IL-2 and IL-4
produced from T cells were evaluated by using the
quantitative sandwich enzyme immunoassay technique.
In response to phytohemagglutinin (PHA)
stimulation, T cells produced increased levels of
IL-2 and IL-4. However, the expressions of both
cytokines were significantly inhibited when PHAactivated
T cells were preexposed to 12.5 g/ml
and 25 g/ml of sonicated E. faecalis extracts (p <
0.05). This effect was concentration-dependent,
because the levels of IL-2 and IL-4 expressions
were not affected by the addition of a low concentration
(5 g/ml) of sonicated extract. These findings
suggest that Th1 and Th2 immunosuppression
mediated by E. faecalis could be a part of the
pathogenic mechanism of the endodontic failure
associated with this microorganism.
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