백서 타액선 선포 세포의 배양

Abstract

We invertigated the culture condition and effects of various growth factors on the culture of salivary gland acinar cells. Male Sprague-Dawley rats (about 6 weeks old) were sacrificed and their submandibular, subingual, and parotid glands were used as specimens. High oxygen level more than 90% and coating of Matrigel on culture dish were important factors to help increase the survival time of acinar cells. Proper concentration of enymes such as collagenase and hyaluronidase during isolation steps was also important. Addition of various growth factors such as dexamethasone, insulin, transferrin, selenous acid, reduced glutathione, epidermal growth factor, isoproterenol, and putrescine in culture medium helped to increase lifetime of cultured salivary acinar cells.