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Expression of thermosensitive two-pore domain K+ channels in human keratinocytes cell line HaCaT cells

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Authors
Kang, Dawon; Kim, Sung-Hee; Hwang, Eun-Mi; Kwon, Oh-Sang; Yang, Hae-Young; Kim, Eun-Sook; Choi, Tae Hyun; Park, Jae-Yong; Hong, Seong-Geun; Han, Jaehee
Issue Date
2007-10-08
Publisher
Wiley-Blackwell
Citation
Exp Dermatol 2007;16:1016-1022
Keywords
keratinocytes; potassium channels; tandem-pore domain; temperature sense
Description
The definitive version is available at www3.interscience.wiley.com.
Abstract
Recent studies have shown that keratinocytes can sense temperature via thermo-transient receptor potential (TRP) channels. It is not known whether other thermosensitive ion channels such as TREK-1, TREK-2 and TRAAK (TREKs ⁄ TRAAK) that are members of the two-pore domain K+ (K2P) channel family are expressed in human keratinocytes. Here, we identified the expression of TREKs ⁄ TRAAK in human keratinocytes-derived cell line HaCaT cells using RT-PCR, immunocytochemistry, Western blot analysis and patch-clamp technique. RT-PCR showed that all six K2P channels tested (TASK-1, TASK-3, TREK-1, TREK-2, TRAAK and TASK-2) were expressed in HaCaT cells, as well as in skin and dorsal root ganglion (DRG) of rat. The expression of TREKs ⁄ TRAAK mRNA identified by RT-PCR was further studied at the protein level. Using anti-TREK-1, -TREK-2 and -TRAAK, bands of 46, 60 and 43 kDa, respectively, were observed at plasma membrane of HaCaT cells. Immunostaining also showed that TREK-1, TREK-2 and TRAAK were expressed in all area of cells including plasma membrane. Whole-cell K+ currents recorded from HaCaT cells were activated by arachidonic acid and heat. These results suggest that TREKs ⁄ TRAAK channels could act as thermosensors in human keratinocytes.
ISSN
0906-6705 (print)1600-0625 (online)
Language
English
URI
http://hdl.handle.net/10371/5324
DOI
https://doi.org/10.1111/j.1600-0625.2007.00626.x
https://doi.org/10.1111/j.1600-0625.2007.00626.x
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College of Medicine/School of Medicine (의과대학/대학원)Plastic Surgery (성형외과학전공)Journal Papers (저널논문_성형외과학전공)
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