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Expression of thermosensitive two-pore domain K+ channels in human keratinocytes cell line HaCaT cells
DC Field | Value | Language |
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dc.contributor.author | Kang, Dawon | - |
dc.contributor.author | Kim, Sung-Hee | - |
dc.contributor.author | Hwang, Eun-Mi | - |
dc.contributor.author | Kwon, Oh-Sang | - |
dc.contributor.author | Yang, Hae-Young | - |
dc.contributor.author | Kim, Eun-Sook | - |
dc.contributor.author | Choi, Tae Hyun | - |
dc.contributor.author | Park, Jae-Yong | - |
dc.contributor.author | Hong, Seong-Geun | - |
dc.contributor.author | Han, Jaehee | - |
dc.date.accessioned | 2009-07-08T04:53:01Z | - |
dc.date.available | 2009-07-08T04:53:01Z | - |
dc.date.issued | 2007-10-08 | - |
dc.identifier.citation | Exp Dermatol 2007;16:1016-1022 | en |
dc.identifier.issn | 0906-6705 (print) | - |
dc.identifier.issn | 1600-0625 (online) | - |
dc.identifier.uri | https://hdl.handle.net/10371/5324 | - |
dc.description | The definitive version is available at www3.interscience.wiley.com. | en |
dc.description.abstract | Recent studies have shown that keratinocytes can sense temperature via thermo-transient receptor potential (TRP) channels. It is not known whether other thermosensitive ion channels such as TREK-1, TREK-2 and TRAAK (TREKs ⁄ TRAAK) that are members of the two-pore domain K+ (K2P) channel family are expressed in human keratinocytes. Here, we identified the expression of TREKs ⁄ TRAAK in human keratinocytes-derived cell line HaCaT cells using RT-PCR, immunocytochemistry, Western blot analysis and patch-clamp technique. RT-PCR showed that all six K2P channels tested (TASK-1, TASK-3, TREK-1, TREK-2, TRAAK and TASK-2) were expressed in HaCaT cells, as well as in skin and dorsal root ganglion (DRG) of rat. The expression of TREKs ⁄ TRAAK mRNA identified by RT-PCR was further studied at the protein level. Using anti-TREK-1, -TREK-2 and -TRAAK, bands of 46, 60 and 43 kDa, respectively, were observed at plasma membrane of HaCaT cells. Immunostaining also showed that TREK-1, TREK-2 and TRAAK were expressed in all area of cells including plasma membrane. Whole-cell K+ currents recorded from HaCaT cells were activated by arachidonic acid and heat. These results suggest that TREKs ⁄ TRAAK channels could act as thermosensors in human keratinocytes. | en |
dc.description.sponsorship | This work was supported by grants numbers RO1-2004-000-10443-0 and R13-2005-012-01002-0 from the Basic Research Program of the Korea Science & Engineering Foundation and the Korea Research Foundation Grant (KRF-2004-005-E00008). | en |
dc.language.iso | en | - |
dc.publisher | Wiley-Blackwell | en |
dc.subject | keratinocytes | en |
dc.subject | potassium channels | en |
dc.subject | tandem-pore domain | en |
dc.subject | temperature sense | en |
dc.title | Expression of thermosensitive two-pore domain K+ channels in human keratinocytes cell line HaCaT cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 강다원 | - |
dc.contributor.AlternativeAuthor | 김성희 | - |
dc.contributor.AlternativeAuthor | 황은미 | - |
dc.contributor.AlternativeAuthor | 권오상 | - |
dc.contributor.AlternativeAuthor | 양해영 | - |
dc.contributor.AlternativeAuthor | 김은숙 | - |
dc.contributor.AlternativeAuthor | 최태현 | - |
dc.contributor.AlternativeAuthor | 박재용 | - |
dc.contributor.AlternativeAuthor | 홍성근 | - |
dc.contributor.AlternativeAuthor | 한재희 | - |
dc.identifier.doi | 10.1111/j.1600-0625.2007.00626.x | - |
dc.identifier.doi | 10.1111/j.1600-0625.2007.00626.x | - |
dc.citation.journaltitle | Experimental dermatology | - |
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