Enhancing Effect of Product of Activated Macrophages upon Resident Peritoneal Macrophages

Abstract

Macrophages having Fe and complement receptors can produce several factors which could activate the lymphocytes. It was assumed that the activated macrophages might secrete certain factors which could activate the resident macrophages. Resident macrophages from rat peritoneal cavity were incubated with two kinds of supernatant obtained from the culture of thioglycollate-elicited macrophages with IgG-coated erythrocytes or complement-coated erythrocytes. Supernatant from the culture of elicited macrophages and complement-coated erythrocytes enhanced the attachment with either IgG-or complement-coated erythrocytes, but supernatant induced by IgG-coated erythrocytes augmented the degree of attachment with IgG-coated erythrocytes only. Any enhancing effect of both supernatants was not observed on the ingestion of IgG-or complement-coated erythrocytes.