S-Space College of Medicine/School of Medicine (의과대학/대학원) Dept. of Medicine (의학과) The Seoul Journal of Medicine The Seoul Journal of Medicine Vol. 27 No.2 (1986)
Biology of Neural Cells in Culture: A Review
배양신경세포의 생물학적 특성
- Kim, Seung U.
- Issue Date
- Seoul J Med, Vol.27 No.2, pp. 97-111
- Neural tissue grown and maintained in vitro provides an opportunity to study
some in situ properties of neurons and glial cells under relatively simple and carefully controlled
conditions. To the extent that a given neural preparation retains properties which approach
those exhibited by the intact brain, tissue (cell) culture provides an important model for the
nervous system, and thus represents a potentially crucial step in system reduction, bringing us
close to the mechanistic explication, at the molecular level, of neurobiological functions.
The term "tissue culture" has been used to denote the in vitro cultivation and maintenance
of living biological units from multicellular organisms. The techniques which have been developed
for this purpose fall into four broad classes:
Organotypic (explant) cultures, which are explanted from specific neuroanatomic loci to
substrates as small tissue fragments.
Dissociated cell cultures, which involve the seeding of enzymatically or mechanically dispersed
cells on various attachment substrates.
Reaggregate cultures, which require re-association of dissociated cells into small aggregates.
PUrified cell populations, which are prepared by the isolation of different cell types by
gradient centrifugation or other separation techniques.