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Comparison of Agar and Agarose Culture Methods for Human Tumor Stem Cell Assay of Gynecologic Tumors
부인과 영역의 악성종양에 있어서 human tumor stem cell assay를 위한 agar배지와 agarose배지의 비교

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Authors
Lee, Hyo Pyo; Cha, Sang Hun
Issue Date
1986-06
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med 1986;27(2):169-172
Keywords
Agar; Agarose; Clone Cells; Gynecologic Tumor
Abstract
The human tumor stem cell assay (HTSCA) has broad research applications in
the field of cell biology as well as in clinical chemotherapy. A prospective clinical trial for
improvement of the in vitro growth rate of gynecologic cancers was done between agar and
agarose culture methods. Specimens from 27 gynecologic tumors were disaggregated by
mechanical and enzymatic methods and assayed in soft agar and agarose culture matrix. 9 of
27 tumors grew in the agar system yielding a cloning success rate of 33.3%. Of 27 specimens,
17 have shown colony growth in the agarose culture matrix showing a cloning success rate of
63.0%. Cloning efficiency in agarose improved approximately 2-fold as compared with that in
agar. The development of a soft agarose assay for gynecologic tumor cells will provide a
possibility of an in vitro technique for predicting in vivo response to anticancer drugs.
ISSN
0583-6802
Language
English
URI
http://hdl.handle.net/10371/5505
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 27 No.2 (1986)
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