Numerical Changes of Immunoglobulin Containing Cells in the Lymph Nodes Draining the Uterus of Pregnant Mice

Abstract

This experiment was performed to detect which IgG subclasses are stimulated by the fetal antigens. The experimental animals were mated syngeneically (female ICR X male ICR) or allogeneically (female ICR X male DDY), and the renal and lumbar lymph nodes draining the uterus were dissected out on the 9th and 18th days of pregnancy. The lymph nodes were stained with methyl green pyronin(MGP) and treated with a immunohistochemical reaction. Then each group was compared with the virgin control group. The following results were obtained: 1. In the group of full term mice which were mated syngeneically, the numbers of the IgG1, IgG2b and IgM containing cells were significantly greater than those of virgin, 2. In the group of mid term mice which were mated allogeneically, the number of the IgG2b containing cells was significantly greater than that of virgin. 3. In the group of full term mice which were mated allogeneically, the numbers of the IgGl, IgG2b, IgG3 and IgM containing cells were significantly greater than those of virgin. 4. In the group of full term mice which were mated allogenecially, the numbers of the IgGl, IgG2b and IgG3 containing cells were significantly greater than those of syngeneic mating. On the basis of the above results, it is suggested that the IgG1, IgG2b and IgG3 were stimulated during pregnancy in mice.