SHERP

Immunocytochemical Observation on Actin in Sensory Epithelium of Rat Macula Utriculi
면역조직화학적(免役組織化學的) 관찰을 통한 흰쥐 내이(內耳) 난형낭(卵形囊)의 「액틴」 분포상(分布像)의 연구

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Authors
Lee, Byung Lan; Seoung, Key June; Cho, Sa Sun; Lee, Kwang Ho; Baik, Sang Ho; Chang, Ka Young
Issue Date
1986-12
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med 1986;27(4):307-312
Keywords
Macula utriculi; Actin; Immunocytochemistry
Abstract
This study was performed to establish the vestibular preparation technique for
immunocytochemical detection of actin in the vestibular hair cells. Frozen sections were
chosen for the detection of actin distribution in the macular sensory epithelium. Quick-freezing
in the primary embedding followed by secondary embedding was used for the proper orientation
of specimens.
Immunohistochemistry by peroxidase-antiperoxidase staining and immunofluorescence demonstrated
actin localization in the neck area of the type I hair cells and in the periphery of
the perinuclear area as well as in the stereocilia and in the cuticular plate. Two possibilities
were suggested: First, immunohistochemically stained area in the neck of the type I hair cells
and peripheral perinuclear portion may represent actin filaments of the synaptic area where
the hair cells and nerve endings contact. Second, G-actin pool might exist around perinuclear
area and react with anti-actin atibody but didn't appear as filamentous area by electron
microscopy.
ISSN
0583-6802
Language
English
URI
http://hdl.handle.net/10371/5520
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 27 No.4 (1986)
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