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Proliferative Activity of Pituitary Mammotrophs in Culture : a Morphological Study on DNA Synthesis Using In Vitro Bromodeoxyuridine Labeling Method

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Authors
Baik, Sang-Ho; Hwang, Se Hee; Lee, Eun-Young; Lee, Byung Lan
Issue Date
1993-12
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med, Vol.34 No.4, pp. 237-243
Keywords
Mummotroph proliferationBromodeoxvuridineRatAnterior pituitary cell cultureDouble inununohistochemistrv
Abstract
The present study was designed to investigate the proliferative activity of
mammotroph in the long-term monolayer cultures of male rat pituitaries by use of
bromodeoxyuridineflsrdl.ll-labeltnq technique combined with double immunohistochemical
staining. Rat anterior pituitary cells were exposed to 100 uM BrdU for 4 hrs at 4,
7, 12, 15, 20, 25 and 30 days in the primary cultures. After fixation with modified
Camoy's fixative, double immunohistochemical staining with anti-BrdU and antiprolactin
antibody was performed. It was shown that the ratio of BrdU-Iabeled
mammotrophs per 100 mammotrophs(BrdU Labeling Index) was 8.2% at 4 day and 8.
0% at 7 day in our cultures. Thereafter, it decreased until 30 days(1.7%). These results
demonstrated that the increase in the proportion of mammotrophs observed in our previous
monolayer cultures is caused, at least in part, by the cell division of mammortrophs.
ISSN
0582-6802
Language
English
URI
http://hdl.handle.net/10371/5570
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 34 No.4 (1993)
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