SubrenaI CapsuIe Tumor ImpIant Assay with Frozen Tumor Tissue: A Preliminary Study Using Mouse Sareoma and Human Uterine Cervix Cancer

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Kang, Soon Beom; Kim, Jong-Hyeok
Issue Date
Seoul National University College of Medicine
Seoul J Med 1993;34(2):131-138
Suhrenal capsule tumor implant assay (SRCA)Chemosensitivity testFrozen tumor tissue
The purpose of this study is to evaluate the possibility of application
of SHeA to previously frozen tumor tissue. Mouse sarcoma-I80 and human squamous
cell carcinoma of the uterine cervix were used as implanting tumor specimens.
The fresh tumor specimens were frozen by rapid freezing method and
stored in a liquid nitrogen tank. On the 7th day of freezing, the specimens were
thawed and diced into 1 cubic millimeter pieces, and implanted beneath the subrenal
capsule of ICH mice. The average viabilities before freezing and after thawing
were 86.7% and 10.5% in mouse sarcoma-I80 respectively. And those of
human uterine cervix cancer were 73.3% and 9.5% respectively. The evaluability
of implanted tumor was 83.3% on the 6th day and 73.7% on the 12th day in
mouse sarcoma, and in human cervix cancer, 75.0% and 66.7% respectively. The
growth of previously frozen implanted mouse sarcoma was significant, 2.1 times
on the 6th day and 73.9 times on the 12th day in volume. In the case of cervix
cancer, the previously frozen tumor implants also grew significantly, 1.7 times on
the 6th day and 2.1 times on the 12th day in volume. In conclusion, this preliminary
laboratory study showed that SHCA could be applied to frozen tumor tissue
as well as to fresh tissue for chemosensitivity test.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 34 No.2 (1993)
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