Browse

A Study on the Mechanism of Myocardial Ischemic-Reperfusion Injury: The Role of Oxygen-free Radicals In Hypoxic-Reoxygenation Injury in Isolated Cardiomyocyte of Rat
허혈심근 재관류 손상의 기전에 관한 연구 : 흰쥐심근 분리세포의 저산소-산소재공급손상에 있어서 산소라디칼의 역할

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Kim, Myung-Suk; Park, Jong-Wan; Kim, Yong-Sik; Chung, Myung-Hee; Lee, Dong-Keun
Issue Date
1989-12
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med, Vol.30 No.4, pp. 231-242
Keywords
Isolated cardiomyocyteReoxygenation injuryOxygen-free radical
Abstract
The possible involvement of intracellulary-producing oxygen-free radiclas in
the genesis of the reperfusion injury of the heart was investigated in preparations of isolated
cardiac myocyte. Calcium-tolerant cardiomyocytes were isolated from an adult rat heart by
treatment with collagenase. The freshly isolated myocyte preparations contained more than
86% of viable cells by trypan blue exclusion criterion, with 84% or more of the cells
showing the elongated rod-shaped morphology of the typical heart cell. The ATP content
was 4.45 p moles/ g wet wt., which was comparable to that of the intact rat heart tissue.
Incubation of the myocyte preparation in hypoxic medium with limited energy substrate and
low calcium produced round-shaped cells associated with a decrease in number of the
typical rod-shaped cells and total viable cells. Reoxygenation of the medium following a
period of hyposix incubation induced a further decrease in viable cell numbers with a
concomitant increase in the release of intracellular enzymes (CPK, LDH) from the myocytes.
The ATP content of the isolated cardiomyocyte rapidly decreased by hyposic incubation, but
this decrease was prevented considerably by reoxygenation. Digitonized cardiomyocyte preparations
incubated with succinate produced a superoxide anion under aerobic conditions as
well as under reoxygenated conditions following hypoxia. The capacity of superoxide anion
production was much more highly maintained during the reoxygenation period than in the
continuous aerobic condition. The cellular activites of defensive enzyme systems against
oxygen free radicals, superoxide dismutase and glutathione peroxidase, were significantly
lowered with hypoxic incubation. It is suggested from this study that durinq reoxygenation
cardiomyocyte, and these radicals may contribute to the development of reoxygenation or
reperfusion injury of the myocardium.
ISSN
0582-6802
Language
English
URI
http://hdl.handle.net/10371/5602
Files in This Item:
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 30 No.4 (1989)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse