SHERP

An Improved Indirect Enzyme-Linked Immunosorbent Assay (ELISA) Suitable for Mass Screening of Anti-Sperm Antibodies
항정자 항체의 검출을 위한 간접 ELISA법의 고안

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Authors
Kim, Jin Q.; Lee, Hee Yong; Kim, Sang In
Issue Date
1989-12
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med 1989;30(4):263-270
Keywords
Antisperm antibodies (ASA)Indirect ELISAVasectomy
Abstract
An improved, indirect enzyme-linked immunosorbent assay (ELISA), which
detects antisperm antibodies (ASA) in serum, which appears to be a sensitive and easy
quantitative procedure for mass screening that is not available with conventional biologic
assay systems. Using known positive human antisperm antisera, it was found that the ELISA
method was quite reproducible at a wide spectrum of titers. When comparing the ELISA
method and the gelatin agglutination (GAT) with the tray agglutination test (TAT) using
heterologous mouse anti-human sperm antisera, it was found that the ELISA method was
more sensitive than the GAT or TAT by more than a three fold dilution factor. The sera of 85
vasectomized subjects were studied with the ELISA, GAT and TAT to determine ASA. We
concluded that, in busy laboratories with heavy workloads, this improved ELISA is a very
sensitive, easily reproducible and efficient method of mass screening for sperm immunity.
ISSN
0583-6802
Language
English
URI
http://hdl.handle.net/10371/5608
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 30 No.4 (1989)
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