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Differential diagnosis of Taenia asiatica using multiplex PCR

Cited 46 time in Web of Science Cited 52 time in Scopus
Authors
Jeon, Hyeong-Kyu; Chai, Jong-Yil; Kong, Yoon; Waikagul, Jitra; Insisiengmay, Bounnaloth; Rim, Han-Jong; Eom, Keeseon S
Issue Date
2008-11-20
Publisher
Elsevier
Citation
Exp Parasitol. 2009 Feb;121(2):151-6. Epub 2008 Nov 5.
Keywords
AnimalsDNA Primers/chemistryDNA, Helminth/*chemistry/isolation & purificationDNA, Mitochondrial/chemistryDiagnosis, DifferentialFeces/parasitologyHumansPolymerase Chain Reaction/*methodsSensitivity and SpecificitySpecies SpecificityTaenia/*classification/geneticsTaeniasis/*diagnosis/parasitology
Abstract
Taenia asiatica and T. saginata are frequently confused tapeworms due to their morphological similarities and sympatric distribution in Asian regions. To resolve this problem, a high-resolution multiplex PCR assay was developed to distinguish T. asiatica infections from infection with other human Taenia tapeworms. For molecular characterization, the species specificity of all materials used was confirmed by sequencing of the cox1 gene. Fifty-two samples were analyzed in this study, comprising 20 samples of T. asiatica genomic DNA from China, Korea, and the Philippines; 24 samples of T. saginata from Belgium, Chile, China, Ethiopia, France, Indonesia, Korea, Laos, the Philippines, Poland, Taiwan, Thailand, and Switzerland; and 10 samples of T. solium from Cape Verde, China, Honduras, and Korea. The diagnostic quality of the results obtained using PCR and species-specific primers designed from valine tRNA and NADH genes was equal to that based on the nucleotide sequencing of the cox1 gene. Using oligonucleotide primers Ta4978F, Ts5058F, Tso7421F, and Rev7915, the multiplex PCR assay was useful for the differentially diagnosing T. asiatica, T. saginata, and T. solium based on 706-, 629-, and 474-bp bands.
ISSN
1090-2449 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19017531

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WFH-4TVHSSJ-1-7&_cdi=6795&_user=168665&_orig=search&_coverDate=02%2F28%2F2009&_sk=998789997&view=c&wchp=dGLbVtz-zSkWA&md5=f175b29e1fc466d08b324b9e5811597f&ie=/sdarticle.pdf

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6WFH-4TVHSSJ-1-7&_cdi=6795&_user=168665&_orig=search&_coverDate=02%2F28%2F2009&_sk=998789997&view=c&wchp=dGLbVlb-zSkzV&md5=f175b29e1fc466d08b324b9e5811597f&ie=/sdarticle.pdf

http://hdl.handle.net/10371/58491
DOI
https://doi.org/10.1016/j.exppara.2008.10.014
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College of Medicine/School of Medicine (의과대학/대학원)Parasitology and Tropical Medicine (기생충학전공)Journal Papers (저널논문_기생충학전공)
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