Effects of the Concentration of Medium Glucose on Glucose Disappearance and Oxidative Metabolism by Liver Slices of Rats

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Chang, Shin Yo; Rhee, Sang Don; Rhee, Ung Sup
Issue Date
Seoul National University College of Medicine
Seoul J Med 1962;3(1):1-7
Liver slices of Sprague-Dawley rat were incubated
with various concentration of 04·glucose in the
incubation medium. A constant condition was main·
tained by periodic replacement of the incubation
medium and gas phase throughout the experimental
period, during whichhourly samples were withdrawn
and analyzed for glucose disappearance rate, and
the rate of oxidation of C14·glucose into CO2, From
32 cases of experiments in which concentrations of
C14·glucose in the incubation medium were varied, the
following results were obtained.
1. Every metabolic rate vs, concentration curve
for glucose disappearance rate, relative specific
activity and C02 production rate from 04'glucose
represents characteristic diphasic nature as in the
process of enzyme reaction, arid approaches to a
plateau at the medium concentration of about 150
2. A plateau value for glucose disappearance was
65,uM/hr/gm, for RSA, 70% and for CO2 production
rate from CI4'glucose, 70,uM/hr/gm. Fractions of
glucose disappeared into CO2 or RGD were average
19% for all ranges of glucose concentrations.
3. Km values for enzyme concerned with glucose
transport at the cell membrane and oxidative
metabolism were 3.33 x 10-4 M/L and 3.62 x 1O-4M/ L
respectively. These Km values imply that glucose oxidation in the metabolic pool would be interfered
by penetration lag at the cell membrane.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 03 No.1 (1962)
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