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Osteogenic Differentiation Potential in Parthenogenetic Murine Embryonic Stem Cells

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Authors
Min, Byung-Moo; Kang, Hoin; Cha, Eun Sook; Choi, Young-Ju; Roh, Sangho
Issue Date
2008-09
Publisher
Korean Acadamy of Oral Biology
Citation
International Journal of Oral Biology 2008;33:91-95
Keywords
embryonic stem cellparthenogenesisosteogenic cell
Abstract
Embryonic stem cells have a pluripotency and a potential
to differentiate to all type of cells. In our previous study, we
have shown that embryonic stem cells (ESCs) lines can be
generated from murine parthenogenetic embryos. This
parthenogenetic ESCs line can be a useful stem cell source
for tissue repair and regeneration. The defect in full-term
development of parthenogenetic ESCs line enables
researchers to avoid the ethical concerns related with ESCs
research. In this study, we presented the results demonstrating
that parthenogenetic ESCs can be induced into
osteogenic cells by supplementing culture media with
ascorbic acid and ß-glycerophosphate. These cells showed
morphologies of osteogenic cells and it was proven by Von
Kossa staining and Alizarin Red staining. Expression of
marker genes for osteogenic cells (osteopontin, osteonectin,
alkaline phosphatase, osteocalcin, bone-sialoprotein, collagen
type1, and Cbfa1) also confirmed osteogenic potential of
these cells. These results demonstrate that osteogenic cells
can be generated from parthenogenetic ESCs in vitro.
ISSN
1226-7155
Language
English
URI
http://hdl.handle.net/10371/61981
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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