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Osteogenic potential of embryonic stem cells in tooth sockets

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Authors
Min, Byung-Moo; Kang, Hyun ki; Roh, Sangho; Lee, Gabsang; Hong, Seong-Doo; Kang, Hoin
Issue Date
2008-05
Publisher
Spandidos Publications
Citation
International Journal of Molecular Medicine 21: 539-544
Keywords
embryonic stem celldifferentiationembryoid bodytransplantiation tooth socketosteoid
Abstract
Embryonic stem cells (ESCs) are established from blastocysts and give rise to various types of cells and tissues. In the present study, we assessed the osteogenic potential of ESCs using in vitro culture conditions and in vivo differentiation in tooth sockets. An ESC-derived embryoid body (EB) was formed and subsequently induced to an osteogenic lineage. The differentiated EB cells exhibited increased expression of various osteogenic markers as determined by real-time PCR analysis. Likewise, the differentiated EB-derived cells had enhanced alkaline phosphatase activity and calcium accumulation, as determined by cytochemical methods. For in vivo transplantation, mixtures of ESCs and hydroxyapatite/ tricalcium phosphate particles or EBs alone were transplanted into female rat tooth sockets. After 12 weeks, we observed formation of osteogenic structure in the tooth sockets without evidence of teratomas. These data suggest that pluripotent ESCs can serve as an alternative source for the reconstruction of craniofacial structures, as well as for further applications
ISSN
1107-3756
Language
English
URI
http://hdl.handle.net/10371/62075
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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