Browse

p44/42 MAPK is necessary for receptor activator of nuclear factor-kB ligand induction by extracellular high calcium

Cited 22 time in Web of Science Cited 23 time in Scopus
Authors
Kim, Gwan-Shik; Kim, Yong Hee; Kim, Jin Mi; Kim, Si Nae; Baek, Jeong-Hwa
Issue Date
2003-05
Publisher
Elsevier
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 304 (2003) 729-735.
Keywords
Extracellular calciumRANKLp44/42 MAPKOsteoblastCalcium-sensing receptor
Abstract
Although extracellular calcium (Ca2+o) has been suggested to modulate bone remodeling, the exact mechanism is unclear. This study was performed to explore the signaling pathways of high Ca2+o that are responsible for controlling the expression of receptor activator of NF-κB ligand (RANKL) in mouse osteoblastic cells. As previously reported, high Ca2+o increased RANKL expression. However, the G protein-coupled Ca2+o-sensing receptor (CaSR) was not detected in the primary cultured mouse osteoblastic cell. The inhibition of the pertussis-sensitive G protein, phospholipase C, protein kinase C, intracellular calcium mobilization, p38 MAPK, or phosphoinositide 3-kinase did not block RANKL induction caused by high Ca2+o. In contrast, the inhibition of p44/42 MAPK pathway reduced the RANKL expression induced by high Ca2+o. Moreover, high Ca2+o activated p44/42 MAPK and MEK1/2. These results suggest that RANKL induction by high Ca2+o might not be mediated by CaSR and its putative downstream signaling pathways, but the pathway employing p44/42 MAPK is involved in the high Ca2+o-induced RANKL expression in mouse osteoblastic cells.
ISSN
0006-291X
Language
English
URI
http://hdl.handle.net/10371/62154
DOI
https://doi.org/10.1016/S0006-291X(03)00661-2
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse