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A new method for rapid screening of bacterial species- or subspecies-specific DNA probes.

Cited 11 time in Web of Science Cited 12 time in Scopus
Authors
Kook, Joong-Ki; Kim, Mi-Kwang; Seong, Jin-Hyo; Kim, Dong-Kie; Kim, Byung-Ock; Park, Joo-Cheol; Kim, Kack-Kyun; Choe, Son-Jin; Min, Byung-Moo
Issue Date
2003-02
Publisher
Federation of European Microbiological Societies
Citation
FEMS Microbiol Lett. 219(1),121-7.
Keywords
Inverted dot blot hybridizationScreening methodDNA probe
Abstract
A simple assay for the rapid screening of bacterial species- or subspecies-specific DNA probes for the random cloning method is presented, involving the use of genomic DNAs as probes and recombinant plasmid DNAs containing genomic DNA digested with Hin dIII as targets. The optimal amount of target DNAs and the concentration of digoxigenin-labeled genomic DNA probes were 20 ng and 100 ng ml−1 (or 10 ng and 200 ng ml−1), respectively. The method was applied to the development of Fusobacterium nucleatum subspecies-specific probes. Our results showed that four out of 96 probes were F. nucleatum subspecies-specific, which was confirmed by Southern blot analysis. Our results indicate that the new method can be used for the rapid screening of species- or subspecies-specific probes.
ISSN
0378-1097
Language
English
URI
http://hdl.handle.net/10371/62227
DOI
https://doi.org/10.1016/S0378-1097(03)00021-1
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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