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Functional expression of thermo-transient receptor potential channels in dental primary afferent neurons: Implication for tooth pain

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Authors
Park, Kyungpyo; Park, Chul-Kyu; Kim, Mi Sun; Fang, Zhi; Li, Hai Ying; Jung, Sung Jun; Choi, Se-Young; Lee, Sung Joong; Kim, Joong Soo; Oh, Seog Bae
Issue Date
2006-06
Publisher
American Society for Biochemistry and Molecular Biology
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY 281, 17304-17311
Abstract
Temperature signaling can be initiated by members of transient receptor potential family (thermo-TRP) channels. Hot and cold substances applied to teeth usually elicit pain sensation. This study investigated the expression of thermo-TRP channels in dental primary afferent neurons of the rat identified by retrograde labeling with a fluorescent dye in maxillary molars. Single cell reverse transcription-PCR and immunohistochemistry revealed expression of TRPV1, TRPM8, and TRPA1 in subsets of such neurons. Capsaicin (a TRPV1 agonist), menthol (a TRPM8 agonist), and icilin (a TRPM8 and TRPA1 agonist) increased intracellular calcium and evoked cationic currents in subsets of neurons, as did the appropriate temperature changes (>43 °, <25 °, and <17 °C, respectively). Some neurons expressed more than one TRP channel and responded to two or three corresponding stimuli (ligands or thermal stimuli). Immunohistochemistry and single cell reverse transcription-PCR following whole cell recordings provided direct evidence for the association between the responsiveness to thermo-TRP ligands and expression of thermo-TRP channels. The results suggest that activation of thermo-TRP channels expressed by dental afferent neurons contributes to tooth pain evoked by temperature stimuli. Accordingly, blockade of thermo-TRP channels will provide a novel therapeutic intervention for the treatment of tooth pain.
ISSN
0021-9258
Language
English
URI
http://hdl.handle.net/10371/62329
DOI
https://doi.org/10.1074/jbc.M511072200
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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