SHERP

Chitosan nano-/microfibrous double-layered membrane with rolled-up three-dimensional structures for chondrocyte cultivation

Cited 0 time in webofscience Cited 34 time in scopus
Authors
Shim, In Kyong; Suh, Won Hee; Lee, Sang Young; Lee, Sang Hoon; Heo, Seong Joo; Lee, Myung Chul; Lee, Seung Jin
Issue Date
2008
Publisher
Wiley-Blackwell
Citation
J Biomed Mater Res 90A:595–602, 2009
Keywords
nanofiberelectrospinningchitosanchondrocytebiomimetic
Abstract
With an aim to mimic natural extracellular matrix, we fabricated the nano- and microfibrous matrix with chitosan by electrospinning nanofibers onto predefined microfibrous mesh for effective chondrocytes cultivation. Rolling the double-layered nano-/microfibrous membranes produced three-dimensional (3-D) scaffolds that exhibited the interconnected open pore structure in their scanning electron microscopy images. In vitro chondrocyte culture experiment showed that this nano-/microfibrous 3-D matrix provided a significantly greater microenvironment for chondrocytes to proliferate and produce glycosaminoglycan as compared with only microfibrous 3-D matrix. This difference could be explained by the result on 2-D membrane, where chitosan nanofibrous surface substantially facilitated the cellular attachment and proliferation, and efficiently prevented phenotypic changes of chondrocytes, when compared with chitosan microfibrous membrane and film. In this regard, the nano-/microfibrous 3-D matrix we fabricated in this study would possess a great potential as a system for effective chondrocyte cultivation and also for application to cartilage regeneration therapy.
ISSN
1549-3296
Language
English
URI
http://hdl.handle.net/10371/62374
DOI
https://doi.org/10.1002/jbm.a.32109
Files in This Item:
There are no files associated with this item.
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse