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Chlorpromazine inhibits store-operated calcium entry and subsequent noradrenaline secretion in PC12 cells.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Choi, Se-Young | - |
dc.contributor.author | Kim, Yong-Hyun | - |
dc.contributor.author | Lee, Yong-Kyu | - |
dc.contributor.author | Kim, Kyong-Tai | - |
dc.date.accessioned | 2010-04-02T01:12:52Z | - |
dc.date.available | 2010-04-02T01:12:52Z | - |
dc.date.issued | 2001 | - |
dc.identifier.citation | British Journal of Pharmacology (2001) 132, 411-418 | en |
dc.identifier.issn | 0007-1188 | - |
dc.identifier.uri | https://hdl.handle.net/10371/62405 | - |
dc.description.abstract | 1 The e ect of chlorpromazine on the store-operated Ca2+ entry activated via the phospholipase C
signalling pathway was investigated in PC12 cells. 2 Chlorpromazine inhibited the sustained increase after the initial peak in the intracellular Ca2+ concentration produced by bradykinin while having no e ect on the initial transient response. The inhibition was lowered by the removal of extracellular free Ca2+. However, chlorpromazine did not inhibit bradykinin-induced inositol 1,4,5-trisphosphate production. 3 Chlorpromazine inhibited the bradykinin-induced noradrenaline secretion in a concentration- dependent manner (IC50: 24+5 mM, n=3). 4 To test for a direct e ect of chlorpromazine on store-operated Ca2+ entry, thapsigargin, an inhibitor of microsomal Ca2+-ATPase, was used to induce store-operated Ca2+ entry in PC12 cells. Chlorpromazine reduced the thapsigargin-induced sustained Ca2+ level (IC50: 24+2 mM, n=3), and the inhibition also occluded the inhibitory action of 1-[-[3-(4-methoxyphenyl) propoxy]-4- methoxyphenyl]-1H-imidazole hydrochloride (SK&F96365). 5 The results suggest that chlorpromazine negatively modulates the store-operated Ca2+ entry activated subsequent to PLC activation. | en |
dc.description.sponsorship | We thank Ms M.J. Kim for her helpful technical assistance. We
are grateful to Ms G. Hoschek for editing this manuscript. This study was supported by grants from the KOSEF, National Research Laboratory Program, and Brain Science and Engineering Research Program sponsored by the Ministry of Science and Technology (1998). This study was also supported by the Korea Research Foundation and the Brain Korea Program from the Ministry of Education. | en |
dc.language.iso | en | en |
dc.publisher | Nature Publishing Group | en |
dc.subject | Chlorpromazine | en |
dc.subject | bradykinin | en |
dc.subject | phospholipase C | en |
dc.subject | store-operated Ca2+ entry | en |
dc.subject | neurotransmitter secretion | en |
dc.title | Chlorpromazine inhibits store-operated calcium entry and subsequent noradrenaline secretion in PC12 cells. | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 최세영 | - |
dc.contributor.AlternativeAuthor | 김용현 | - |
dc.contributor.AlternativeAuthor | 이용규 | - |
dc.contributor.AlternativeAuthor | 김경태 | - |
dc.identifier.doi | 10.1038/sj.bjp.0703840. | - |
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