S-Space College of Medicine/School of Medicine (의과대학/대학원) Laboratory Medicine (검사의학전공) Journal Papers (저널논문_검사의학전공)
Comparison of the MicroScan, VITEK 2, and Crystal GP with 16S rRNA sequencing and MicroSeq 500 v2.0 analysis for coagulase-negative Staphylococci
- Kim, Miyoung; Heo, Se Ran; Choi, Soon Hee; Kwon, Hyelin; Park, Jeong Su; Seong, Moon-Woo; Lee, Do-Hoon; Park, Kyoung Un; Song, Junghan; Kim, Eui-Chong
- Issue Date
- BioMed Central
- BMC Microbiol. 2008 Dec 23;8:233.
- Bacteriological Techniques/*methods/*standards; Coagulase/metabolism; Phenotype; RNA, Ribosomal, 16S/genetics; Reproducibility of Results; Staphylococcal Infections/microbiology; Staphylococcus/*classification/enzymology/genetics/*physiology
- BACKGROUND: Three phenotypic identification systems (MicroScan, VITEK 2, and Crystal GP) were evaluated for their accuracy to identify coagulase-negative staphylococci (CNS). A total of 120 clinical isolates confirmed to be CNS via 16S rRNA sequencing and analysis with the MicroSeq 500 v2.0 database were assessed. RESULTS: The MicroScan, VITEK 2, and Crystal GP systems correctly identified 82.5%, 87.5%, and 67.5% of the isolates, respectively. Misidentification was the main problem in MicroScan (10.8%) and Crystal GP (23.3%) systems, whereas the main problem of VITEK 2 was low-level discrimination (7.5%). CONCLUSION: None of the 3 phenotypic systems tested could accurately and reliably identify CNS at the species level. Further verifications such as biochemical testing or 16S rRNA sequencing together with analysis using a comparable database might be helpful in this regard.
- 1471-2180 (Electronic)