Deuterium Oxide의 흰쥐 복막강내 이동

Abstract

injected into the peritoneal cavity of unanesthetized female rats, and the distribution of it was measured in plasma, intestinal wall, intestinal content, liver, kidney, heart and in the skeletal muscle. Animals were sacrificed at 3 min., 6 min.. 12 min. or 30 min. after the injection. In 6 animals the extent of the diffusion of DzO, injected after death, in the abdominal organs in the period of 10 minutes was examined. The number of animals used for present study totalled 30. The results obtained were as follows: 1. When DzO of 0.75 ml was injected it was removed from the peritoneal cavity mostly by the mean of the circulation of blood or lymph. Direct diffusion to the abdominal organs seemed to be scanty. 2. In the rats receiving 1.5 ml of DzO the kidney, heart and skeletal muscle owed their content of the test substance to the general circulation. On the other hand, intestinal wall, intestinal content and the liver were predominantly supplied by direct diffusion from the peritoneal cavity. 3. By injection of 3 ml of DzO it promptly diffused into the organs as well as into the intestinal content, except to the skeletal muscle. Circulation washed out D2O thereafter. 4. The surface of all abdominal organs seemed to serve as a diffusible surface for water and it was also postulated that there would be massive exchange of solutes between organs and the peritoneal cavity. 5. Rapid penetration of DzO into intestinal content was in accord with the quick mixing of DzO within the ascitic and edematous depot. 6. It seems to be rational to put DzO in the peritoneal cavity of the animal for the measurment of the total body water by the dilution method.