SHERP

Molecular Cloning of cDNA for Human Epinephrine Synthesizing Enzyme, Phenylethanolamine N-Methyltransferase(PNMT)
인간 Epinephrine합성효소 Phenylethanolamine N-methyltransferase cDNA의 유전자 크로닝

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Authors
Suh, Yoo-Hun; Chun, Yang Sook; Kim, Sung Soo; Park, Chan-Woong
Issue Date
1988-09
Publisher
Seoul National University College of Medicine
Citation
Seoul J Med 1988;29(3):245-251
Keywords
Human libraryλgt10Molecular cloningPNMT
Abstract
An oligo(dT)-primed eDNA was constructed from poly(A)+ -containinq RNA
from human adrenal tissues. The eDNA was inserted into the EcoRl site of ). gtlO phage and
the human library was screened for clones containing cDNAs coding for human phenylethanolamine
N- methyl transferase(PNMT).
Approximately 1.5 X 106 recombinant phage plaques were transferred to nitrocellulose
filters and a eDNA clone was isolated and identified from the ). gtlO DNA by digestion with
EcoRl and was found to .be approximately 1150 base pairs in length containing one internal
EcoRl site. Northern blot analysis showed that the eDNA strongly hybridized to an RNA
species of approximately 1.1 kb, a size appropriate for an mRNA coding for PNMT. The
human adrenal mRNA selected by the eDNA codes for a 31k dalton protein.
This is the first report of the cloning of eDNA for human epinephrine synthesizing enzyme,
PNMT.
ISSN
0583-6802
Language
English
URI
http://hdl.handle.net/10371/6390
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 29 No.3 (1988)
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