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Chitosan-graft-polyethylenimine as a gene carrier
DC Field | Value | Language |
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dc.contributor.author | Jiang, Hu-Lin | - |
dc.contributor.author | Kim, You-Kyoung | - |
dc.contributor.author | Arote, Rohidas | - |
dc.contributor.author | Nah, Jae-Woon | - |
dc.contributor.author | Cho, Myung-Haing | - |
dc.contributor.author | Choi, Yun-Jaie | - |
dc.contributor.author | Akaike, Toshihiro | - |
dc.contributor.author | Cho, Chong-Su | - |
dc.date.accessioned | 2009-08-07T02:44:34Z | - |
dc.date.available | 2009-08-07T02:44:34Z | - |
dc.date.created | 2018-04-11 | - |
dc.date.issued | 2007-02 | - |
dc.identifier.citation | Journal of Controlled Release, Vol.117 No.2, pp.273-280 | - |
dc.identifier.issn | 0168-3659 | - |
dc.identifier.other | 31184 | - |
dc.identifier.uri | https://hdl.handle.net/10371/6467 | - |
dc.description.abstract | Chitosans have been proposed as biocompatible alternative cationic polymers that are suitable for non-viral delivery. However, the transfection efficiency of chitosan-DNA nanoparticles is still very low. To improve transfection efficiency, we prepared chitosan-graft-polyethylenimine (CHI-g-PEI) copolymer by an imine reaction between periodate-oxidized chitosan and polyethylenimine (PEI). The molecular weight and composition of the CHI-g-PEI copolymer were characterized, using multi-angle laser scattering (GPC-MALS) and H-1 nuclear magnetic resonance (H-1 NMR), respectively. The copolymer was complexed with plasmid DNA (pDNA) in various copolymer/DNA (N/P) charge ratios, and the complex was characterized. CHI-g-PEI showed good DNA binding ability and high protection of DNA from nuclease attack. Also, with an increase in charge ratio, the sizes of the CHI-g-PEI/DNA complex showed a tendency to decrease, whereas the zeta potential of the complex showed an increase. The CHI-g-PEI copolymer had low cytotoxicity, compared to PEI 25K from cytotoxicity assays. At high N/P ratios, the CHI-g-PEI/DNA complex showed higher transfection efficiency than PEI 25K in HeLa, 293T and HepG2 cell lines. Our results indicate that the CHI-g-PEI copolymer has potential as a gene carrier in vitro. (c) 2006 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | en |
dc.publisher | Elsevier BV | - |
dc.title | Chitosan-graft-polyethylenimine as a gene carrier | - |
dc.type | Article | - |
dc.contributor.AlternativeAuthor | 조명행 | - |
dc.identifier.doi | 10.1016/j.jconrel.2006.10.025 | - |
dc.citation.journaltitle | Journal of Controlled Release | - |
dc.identifier.wosid | 000244624100016 | - |
dc.identifier.scopusid | 2-s2.0-33846471958 | - |
dc.citation.endpage | 280 | - |
dc.citation.number | 2 | - |
dc.citation.startpage | 273 | - |
dc.citation.volume | 117 | - |
dc.identifier.sci | 000244624100016 | - |
dc.description.isOpenAccess | N | - |
dc.contributor.affiliatedAuthor | Cho, Myung-Haing | - |
dc.contributor.affiliatedAuthor | Choi, Yun-Jaie | - |
dc.contributor.affiliatedAuthor | Cho, Chong-Su | - |
dc.type.docType | Article | - |
dc.description.journalClass | 1 | - |
dc.subject.keywordPlus | LOW-MOLECULAR-WEIGHT | - |
dc.subject.keywordPlus | TRANSFECTION EFFICIENCY | - |
dc.subject.keywordPlus | GLYCOL) COPOLYMERS | - |
dc.subject.keywordPlus | NONVIRAL VECTOR | - |
dc.subject.keywordPlus | DNA DELIVERY | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | POLY(ETHYLENIMINE) | - |
dc.subject.keywordPlus | COMPLEXES | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | ACID | - |
dc.subject.keywordAuthor | non-viral gene delivery | - |
dc.subject.keywordAuthor | chitosan | - |
dc.subject.keywordAuthor | polyethylenimine | - |
dc.subject.keywordAuthor | chitosan-graft-PEI | - |
dc.subject.keywordAuthor | cytotoxicity | - |
dc.subject.keywordAuthor | transfection efficiency | - |
dc.subject.keywordAuthor | gene therapy | - |
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