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Gene Expression Profile by 2,3,7,8-Tetrachlorodibenzo-p-Dioxin in the Liver of Wild-Type (AhR+/+) and Aryl Hydrocarbon Receptor-Deficient (AhR-/-) Mice

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Authors
Yoon, Chang Yong; Park, Misun; Kim, Bang Hyun; Park, Ji Yeon; Park, Mun Suk; Jeong, Youn Kyoung; Kwon, Hyugsung; Jung, Hai Kwan; Kang, HoIl; Lee, Yong Soon; Lee, Beom Jun
Issue Date
2006
Publisher
Japanese Society of Veterinary Science
Citation
J. Vet. Med. Sci. 68: 663-668
Keywords
gene expressionlivermicroarrayTCDD
Abstract
,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is one of the most toxic environmental pollutants that cause various biological effects on mammals. The purpose of our study was to identify the genes involved in hepatotoxicity and hepatocarcinogenesis caused by TCDD. C57BL/6 (AhR+/+, wild type) and B6.129-AhR/J (AhR-/-, knock out) mice were injected i.p. with a single treatment of TCDD at the dose of 100 microg/kg body weight. Relative liver weight was significantly increased at 72 hr after TCDD treatment without an apparent histopathological change in AhR+/+ mice (p<0.05). TCDD treatment also significantly increased activity of serum alanine aminotransferase in AhR-/- mice (p<0.05). The liver was analyzed for gene expression profiles 72 hr later. As compared with AhR-/- mice, the expression of 51 genes (>3-fold) was changed in AhR+/+ mice; 28 genes were induced, while 23 genes were repressed. Most of the genes were associated with chemotaxis, inflammation, carcinogenesis, acute-phase response, immune responses, cell metabolism, cell proliferation, signal transduction, and tumor suppression. This study suggests that the microarray analysis of genes in the liver of AhR+/+ and AhR-/- mice may help to clarify the mechanism of AhR-mediated hepatotoxicity and hepatocarcinogenesis by TCDD.
ISSN
0916-7250 (print)
1347-7439 (online)
Language
English
URI
http://hdl.handle.net/10371/6492
DOI
https://doi.org/10.1292/jvms.68.663
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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