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Characterization of Chemisorbed Hyaluronic Acid Directly Immobilized on Solid Substrates

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Authors
Suh, Kahp Y.; Yang, Jen Min; Khademhosseini, Ali; Berry, David; Tran, Thanh-Nga T.; Park, Hyongshin; Langer, Robert
Issue Date
2005-02-15
Publisher
John Wiley & Sons
Citation
J Biomed Mater Res Part B: Appl Biomater 2005;72B:292-298
Keywords
hyaluronic aciddirect immobilizationcharacterizationchemisorptionsubstratesRAY PHOTOELECTRON-SPECTROSCOPYMOLECULAR-BASISPOLYSACCHARIDESURFACESRECOGNITIONPOLYSTYRENEDEGRADATIONDERIVATIVES
Abstract
Hyaluronic acid (HA) has a number of potential biomedical applications in drug delivery and tissue engineering. For these applications, a prerequisite is to understand the characteristic of HA films directly immobilized to solid substrates. Here, we demonstrate that high molecular weight HA can be directly immobilized onto hydrophilic substrates without any chemical manipulation, allowing for the formation of an ultrathin chemisorbed layer. Hyaluronic acid is stabilized on these surfaces through hydrogen bonding between the hydrophilic moieties in HA [such as carboxylic acid (-COOH) or hydroxyl (-OH) groups] with silanol (-SiOH), carboxylic acid or hydroxyl groups on the hydrophilic substrates. Despite the water solubility, the chemisorbed HA layer remained stable on glass or silicon oxide substrates for at least 7 days in phosphate-buffered saline. Furthermore, HA immobilized on silicon and other dioxide surfaces in much higher quantities than other polysaccharides including dextran sulfate, heparin, heparin sulfate, chondroitin sulfate, dermatan sulfate, and alginic acid. This behavior is related to the molecular entanglement and intrinsic stiffness of HA as a result of strong internal and external hydrogen bonding as well as high molecular weight. These results demonstrate that HA can be used to coat surfaces through direct immobilization.
ISSN
1552-4973
Language
English
URI
http://hdl.handle.net/10371/6536
DOI
https://doi.org/10.1002/jbm.b.30152
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College of Engineering/Engineering Practice School (공과대학/대학원)Dept. of Mechanical Aerospace Engineering (기계항공공학부)Journal Papers (저널논문_기계항공공학부)
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